Effects of coat protein mutations and reduced movement protein expression on infection spread by cowpea chlorotic mottle virus and its hybrid derivatives

Virology
W De JongP Ahlquist

Abstract

Previously we have reported that the essential 3a movement gene of icosahedral cowpea chlorotic mottle virus (CCMV) can be functionally replaced by the 30-kDa movement gene of rod-shaped sunn-hemp mosaic virus (SHMV). Because plant RNA viruses differ in requiring or not requiring coat protein for systemic infection, we have now investigated whether systemic spread by this CCMV/SHMV hybrid is dependent on its CCMV coat protein as well as its SHMV movement protein. We find that either deletion or frameshift mutations in the coat protein gene block systemic spread. Thus, like wild-type CCMV, systemic infection by the hybrid is dependent on both movement protein and coat protein. These results further support the conclusion that the required functions of the coat and movement proteins in CCMV spread do not depend on sequence-specific interaction between these proteins. Additional features of the hybrid also motivated testing the effects of modulating movement protein expression. Creating an extra, out-of-frame translational start codon (AUG) shortly upstream of the 3a movement protein gene in CCMV downregulated its expression 18-fold. Nevertheless, for CCMV derivatives bearing either the CCMV 3a gene or the SHMV 30-kDa gene, the ex...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·W De Jong, P Ahlquist
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·M Kozak
Dec 1, 1990·Virology·I T Petty, A O Jackson
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I T PettyA O Jackson
Mar 1, 1987·American Heart Journal·K Ausubel, B Gitler
May 1, 1993·Journal of Virology·K MiseP Ahlquist

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