Effects of cobalt and chromium ions on oxidative stress and energy metabolism in macrophages in vitro

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Zeina SalloumIsabelle Catelas

Abstract

Cobalt and chromium ions released from cobalt-chromium-molybdenum (CoCrMo)-based implants are a potential health concern, especially since both ions have been shown to induce oxidative stress in macrophages, the predominant immune cells in periprosthetic tissues. Ions of other transition metals (Cd, Ni) have been reported to inhibit the activity of mitochondrial enzymes in the electron transport chain. However, the effects of Co and Cr ions on the energy metabolism of macrophages remain largely unknown. The objective of the present study was to analyze the effects of Co2+ and Cr3+ on oxidative stress and energy metabolism in macrophages in vitro. RAW 264.7 murine macrophages were exposed to 6-18 ppm Co2+ or 50-150 ppm Cr3+ . Results showed a significant increase in two markers of oxidative stress, reactive oxygen species level and protein carbonyl content, with increasing concentrations of Co2+ , but not with Cr3+ . In addition, oxygen consumption rates (OCR; measured using an extracellular flux analyzer) showed significant decreases in both mitochondrial respiration and non-mitochondrial oxygen consumption with increasing concentrations of Co2+ , but not with Cr3+ . OCR results further showed that Co2+ , but not Cr3+ , induced...Continue Reading

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Citations

Aug 20, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Qiong WangRizhi Wang
Apr 16, 2020·Environmental Microbiology·Todsapol TechoChoowong Auesukaree
May 29, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Zeina SalloumIsabelle Catelas
Aug 17, 2019·Frontiers in Bioengineering and Biotechnology·Felipe EltitRizhi Wang
May 15, 2020·Biological Trace Element Research·Ivelin VladovYordanka Gluhcheva
Dec 21, 2020·Chemosphere·Benfeng CaoLiegang Liu
Apr 1, 2021·Environmental Science and Pollution Research International·Qingqing ZhuXinli Li

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