PMID: 20650102Aug 1, 1995Paper

Effects of cobalt on mouse neuroblastoma cells cultured in vitro

Toxicology in Vitro : an International Journal Published in Association with BIBRA
G RepettoM Repetto

Abstract

To compare the effects of cobalt at different cellular levels, cultured mouse neuroblastoma cells (Neuro-2a) were exposed for 24 hr to cobalt(II) chloride. The following toxicity indicators were assessed: cell proliferation by quantification of total protein content; cytoplasmic membrane integrity to cytosolic lactate dehydrogenase leakage; lysosomal hexosaminidase release; lactate dehydrogenase activity (LDH); mitochondrial succinate dehydrogenase activity (SDH); relative neutral red uptake by lysosomes (RNRU); lysosomal hexosaminidase activity (HEX), and acetylcholinesterase activity (AChE). The effect of cobalt on the various indicators differed. Cobalt was not very toxic to neuroblastoma cell proliferation (EC(50) = 200 mum). Cytoplasmic membrane permeability was not specifically increased, and LDH leakage occurred only at high concentrations, prior to the stimulation of HEX activity, an enzyme involved in sphingolipid degradation. In contrast, cobalt was lysosomotropic, with HEX release. The effects on lysosomal function were also studied with the RNRU, showing stimulation at low concentrations and inhibition at high concentrations. Neural AChE was decreased after an initial stimulation at low concentrations. LDH and SDH i...Continue Reading

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Citations

Sep 21, 2000·Toxicology Letters·P da Silva MeloM Haun
Mar 11, 2008·Ecotoxicology and Environmental Safety·Elisa MaranMaría José Ruiz
May 29, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jun ZhongNaoki Matsuda
Mar 10, 2020·Journal of Applied Toxicology : JAT·Clara L Porfírio-DiasJulio C Pieczarka

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