Effects of Coculture With Immune Cells on the Developmental Competence of Mouse Preimplantation Embryos in Vitro and in Utero

Reproductive Sciences
Jaewang LeeJin Hyun Jun

Abstract

The aim of this study was to establish a coculture system using immune cells as well as an in vitro model for inflammatory conditioning using RAW 264.7 mouse macrophages activated by lipopolysaccharide. The direct and indirect coculture systems were applied to evaluate the influence of embryo-to-cell direct or indirect secretory molecules from the cocultured cells. Blastulation rate in vitro (94.6% vs 76.9%, P < .05) and implantation rate in utero (43.3% vs 17.6%, P < .01) were significantly increased in direct coculture with activated RAW 264.7 cells compared to control. We also found the embryotrophic effects in vitro in the indirect coculture system. Our results indicate that coculture of mouse preimplantation embryos with immune cells could improve the developmental competence in vitro and in utero. Taken together, RAW 264.7 cells secret embryotrophic molecules, and it suggests the valuable insights that immune cells could improve in vitro culture conditions of preimplantation embryos.

References

Nov 1, 1993·Journal of Reproduction and Fertility·M T McMasterG K Andrews
Oct 1, 1993·Current Opinion in Obstetrics & Gynecology·A Bongso, C Y Fong
Mar 1, 1997·Electrophoresis·M A ReymondF Köckerling
Jun 17, 2000·Fertility and Sterility·D K GardnerW B Schoolcraft
May 9, 2003·Reproductive Biomedicine Online·Catherine RacowskyKatharine V Jackson
Mar 25, 2004·Molecular Reproduction and Development·Jia-Sen XuWilliam S B Yeung
Jun 29, 2004·Archives of Gynecology and Obstetrics·Kaushik DebYogesh Kumar Jaiswal
May 20, 2005·Reproductive Biomedicine Online·A A Fouladi-NashtaR Webb
May 9, 2007·Infectious Diseases in Obstetrics and Gynecology·Yogesh Kumar JaiswalKaushik Deb
Aug 20, 2008·Fertility and Sterility·Yogesh Kumar JaiswalMadan Mohan Chaturvedi
Feb 4, 2010·The Journal of Clinical Investigation·Yasushi HirotaSudhansu K Dey
Jun 23, 2011·Journal of Cellular and Molecular Medicine·Anika Eva WagnerGerald Rimbach
Dec 3, 2011·American Journal of Reproductive Immunology : AJRI·Maria L ZenclussenAna C Zenclussen
Dec 21, 2011·Journal of Neuroscience Methods·Maren Geissler, Andreas Faissner
Apr 24, 2012·The Journal of Reproduction and Development·Xing-Hui ShenNam-Hyung Kim
Jul 20, 2012·Zygote : the Biology of Gametes and Early Embryos·Varkha AgrawalYogesh Kumar Jaiswal
Nov 7, 2012·Endocrine Regulations·K SmolikovaS Scsukova
May 17, 2013·Biology of Reproduction·Mukesh Kumar JaiswalYogesh Kumar Jaiswal
Jul 23, 2013·The Journal of Clinical Investigation·Alison S CareSarah A Robertson
Jun 3, 2014·Fertility and Sterility·Daniela Galliano, Antonio Pellicer

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Methods Mentioned

BETA
PCR
Electrophoresis
Assay
fluorescence microscopy
enzyme-linked immunosorbent assay

Software Mentioned

Vilber Lourmat
Gel
Doc

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