Effects of cold acclimation and dsRNA injections on Gs1l gene splicing in Drosophila montana

Scientific Reports
David HopkinsMaaria Kankare

Abstract

Alternative splicing, in which one gene produce multiple transcripts, may influence how adaptive genes respond to specific environments. A newly produced transcriptome of Drosophila montana shows the Gs1-like (Gs1l) gene to express multiple splice variants and to be down regulated in cold acclimated flies with increased cold tolerance. Gs1l's effect on cold tolerance was further tested by injecting cold acclimated and non-acclimated flies from two distantly located northern and southern fly populations with double stranded RNA (dsRNA) targeting Gs1l. While both populations had similar cold acclimation responses, dsRNA injections only effected the northern population. The nature of splicing expression was then investigated in the northern population by confirming which Gs1l variants are present, by comparing the expression of different gene regions and by predicting the protein structures of splices using homology modelling. We find different splices of Gs1l not only appear to have independent impacts on cold acclimation but also elicit different effects in populations originating from two very different environments. Also, at the protein level, Gs1l appears homologous to the human HDHD1A protein and some splices might produce f...Continue Reading

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Citations

Jan 30, 2019·The Journal of Experimental Biology·Timothy M Healy, Patricia M Schulte

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Methods Mentioned

BETA
PCR
environmental stress

Software Mentioned

Gene
NORMA
R
BioEdit
Sequence Analysis
Blast search
Modeller
NetPrimer
Malign
in Bodil Modeling Environment

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