Effects of cold and warm ischemia on the mitochondrial oxidative phosphorylation of swine lung

K WilletF E Sluse


The aim of the study was to investigate the consequence of warm and cold ischemia on lung mitochondria in order to define bioenergetic limits within lung could be suitable for pulmonary transplantation. Twenty-two pigs underwent lung harvesting after lung flush with Euro-Collins solution. Mitochondria were isolated from fresh lungs, from lungs submitted to 24 or 48 hr of cold ischemia, to 30 or 45 min of warm ischemia, and to 30 min of warm ischemia followed by 24 or 48 hr of cold ischemia. Mitochondrial oxidative phosphorylation parameters were determined in isolated mitochondria by in vitro measurement of oxygen consumption. Relative to controls, mitochondria submitted to cold ischemia showed an alteration in the oxidoreductase activities of the respiratory chain but no membrane permeability alteration. After 48 hr of cold ischemia, there was a decrease in the yield of the oxidative phosphorylation. Thirty minutes of warm ischemia did not alter the mitochondrial respiratory parameters. However, lung submitted to 45 min of warm ischemia showed mitochondrial damage as a decrease in the oxidative phosphorylation efficiency and ADP availability but no change in the oxidoreductase activities. Relative to cold ischemia alone, 30 mi...Continue Reading


Sep 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S K KimJ H Southard
Dec 1, 1991·Journal of Molecular and Cellular Cardiology·G S Sandhu, G K Asimakis
Jan 1, 1990·Free Radical Research Communications·H Nohl
Aug 1, 1993·Journal of Molecular and Cellular Cardiology·S P AllenD Stone
Apr 15, 1995·Transplantation·A M D'AlessandroF O Belzer
Aug 1, 1994·The American Journal of Physiology·T E GunterC E Gavin
Sep 1, 1993·Basic Research in Cardiology·M Crompton, L Andreeva
May 1, 1993·Biochemical Society Transactions·A P HalestrapC P Connern
Jun 1, 1993·The Annals of Thoracic Surgery·T M EganB R Wilcox
Jan 1, 1995·Acta Anaesthesiologica Scandinavica. Supplementum·N E Saris, K O Eriksson
Apr 15, 1996·Transplantation·M H HenningtonT M Egan
Jul 1, 1996·The Annals of Thoracic Surgery·R J NovickJ Lee
Jul 1, 1996·The Annals of Thoracic Surgery·M C MauneyC G Tribble
Apr 15, 1997·Transplantation·G Kootstra
Oct 6, 1997·The Annals of Thoracic Surgery·D E Van RaemdonckT E Lerut
Dec 22, 1998·Annals of Surgery·D E Van RaemdonckT E Lerut

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