Effects of cold and warm ischemia on the mitochondrial oxidative phosphorylation of swine lung

Transplantation
K WilletF E Sluse

Abstract

The aim of the study was to investigate the consequence of warm and cold ischemia on lung mitochondria in order to define bioenergetic limits within lung could be suitable for pulmonary transplantation. Twenty-two pigs underwent lung harvesting after lung flush with Euro-Collins solution. Mitochondria were isolated from fresh lungs, from lungs submitted to 24 or 48 hr of cold ischemia, to 30 or 45 min of warm ischemia, and to 30 min of warm ischemia followed by 24 or 48 hr of cold ischemia. Mitochondrial oxidative phosphorylation parameters were determined in isolated mitochondria by in vitro measurement of oxygen consumption. Relative to controls, mitochondria submitted to cold ischemia showed an alteration in the oxidoreductase activities of the respiratory chain but no membrane permeability alteration. After 48 hr of cold ischemia, there was a decrease in the yield of the oxidative phosphorylation. Thirty minutes of warm ischemia did not alter the mitochondrial respiratory parameters. However, lung submitted to 45 min of warm ischemia showed mitochondrial damage as a decrease in the oxidative phosphorylation efficiency and ADP availability but no change in the oxidoreductase activities. Relative to cold ischemia alone, 30 mi...Continue Reading

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Citations

Feb 8, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Gina L J Galli, Jeffrey G Richards
Jun 6, 2012·Transplantation Proceedings·O DetryM Meurisse
Jul 31, 2013·Transplant International : Official Journal of the European Society for Organ Transplantation·Julien PottecherPierre Diemunsch
Dec 7, 2000·Biochimica Et Biophysica Acta·K WilletF E Sluse
Jul 16, 2005·International Journal of Cardiology·António AscensãoJosé A Duarte
Sep 16, 2021·Science Translational Medicine·Aadil AliMarcelo Cypel

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