Effects of cold exposure on metabolites in brown adipose tissue of rats

Molecular Genetics and Metabolism Reports
Yuka HiroshimaYasuo Shinohara

Abstract

Brown adipose tissue (BAT) plays an important role in regulation of energy expenditure while adapting to a cold environment. BAT thermogenesis depends on uncoupling protein 1 (UCP1), which is expressed in the inner mitochondrial membranes of BAT. Gene expression profiles induced by cold exposure in BAT have been studied, but the metabolomic biological pathway that contributes to the activation of thermogenesis in BAT remains unclear. In this study, we comprehensively compared the relative levels of metabolites between the BAT of rats kept at room temperature (22 °C) and of those exposed to a cold temperature (4 °C) for 48 h using capillary electrophoresis (CE) time-of-flight mass spectrometry (TOFMS) and liquid chromatography (LC)-TOFMS. We identified 218 metabolites (137 cations and 81 anions) by CE-TOFMS and detected 81 metabolites (47 positive and 34 negative) by LC-TOFMS in BAT. We found that cold exposure highly influenced the BAT metabolome. We showed that the cold environment lead to lower levels of glycolysis and gluconeogenesis intermediates and higher levels of the tricarboxylic acid (TCA) cycle metabolites, fatty acids, and acyl-carnitine metabolites than control conditions in the BAT of rats. These results indicate ...Continue Reading

Citations

Jul 10, 2019·Frontiers in Endocrinology·Elena ZoicoMauro Zamboni
Sep 10, 2020·Electrophoresis·Wei Zhang, Rawi Ramautar
Jul 3, 2020·Current Pharmaceutical Design·Juan SalazarValmore Bermúdez
Sep 24, 2020·Journal of Pharmaceutical and Biomedical Analysis·Katarzyna MiniewskaMichal Ciborowski
May 25, 2021·The FEBS Journal·Zhichao WangLei Jiang
Oct 19, 2019·Analytical Chemistry·Carolina González-RianoCoral Barbas

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

GraphPad Prism
MasterHands
Dual Scan
VANTED ( Visualization and Analysis of Networks containing Exp...

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