Effects of cold shock on microtubule organization and cell cycle in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus)

Marine Biotechnology
Xiang-Ping ZhuJian-He Xu

Abstract

Cytological changes and subsequent mitotic processes were studied in gynogenetically activated eggs of olive flounder subjected to cold-shock treatment using indirect immunofluorescence staining of isolated blastodisks. Obvious differences between controls and treated eggs were detected during early cell division. The developmental process of haploid control was similar to that of the diploid control except several minutes delayed. Spindles disassembled by the cold-shock treatment regenerated soon after treatment, resulting in the occurrence of the first mitosis. The immature daughter centriole was easily depolymerized by cold-shock treatment, leading to the formation of the bipolar spindle in the first cell cycle and the formation of the monopolar spindle in the second cell cycle, resulting in chromosome set doubling. Some two-cell stage eggs had a monopolar spindle in one blastomere and a bipolar spindle in another during the second mitosis. These eggs had a high potency developing into haploid-diploid mosaics. To the best of our knowledge, this study is the first to clarify the mechanism of chromosome set doubling in marine fishes and provides a preliminary cytological basis for developing a reliable and efficient protocol f...Continue Reading

References

Dec 1, 1985·The Journal of Experimental Zoology·K NaruseN Egami
Dec 1, 1981·The Journal of Cell Biology·R Kuriyama, G G Borisy
Nov 1, 1993·Zygote : the Biology of Gametes and Early Embryos·P L Hertzler, W H Clark
Mar 29, 2001·Cell Motility and the Cytoskeleton·A RousseletM Bornens
Sep 14, 2001·Reproduction in Domestic Animals = Zuchthygiene·I PaschosE Kolettas
Sep 6, 2002·Oncogene·Kenji Fukasawa
Feb 13, 2004·Marine Biotechnology·D L MitchellR B Walter

❮ Previous
Next ❯

Citations

Jan 23, 2016·Zygote : the Biology of Gametes and Early Embryos·Jilun HouHaijin Liu

❮ Previous
Next ❯