Effects of collagen IV on neuroblastoma cell matrix-related functions

Experimental Cell Research
Athina K TziniaE C Tsilibary

Abstract

Integrin-mediated interactions with collagen IV and its domains were examined in a human neuroblastoma cell line (SK-N-SH). By adhesion assays we demonstrated that neuroblastoma cells bound to solid-phase intact collagen IV and synthetic cell-binding peptide HEP-III, derived from the collagenous part of the molecule, but not to the main noncollagenous NC1 domain or to the synthetic cell-binding peptide HEP-I, derived from this domain. Monoclonal antibodies against beta1, alpha3, and alpha(v)beta3 integrins resulted in inhibition of cell binding to collagenous substrates by 95, 30, and 35%, respectively. By flow cytometry and immunoblotting it was shown that culture of SK-N-SH cells on collagen IV resulted in alteration in the expression of major neuroblastoma cell integrins. Binding to collagen IV induced the expression and activation of matrix metalloproteinases A and B (MMP-2, MMP-9), with a concomitant increase at the protein level of tissue-specific inhibitors of metalloproteinases (TIMP-1, TIMP-2). Finally, the expression of MMP-2 was significantly up-regulated by anti-alpha3beta1 antibodies, whereas ligation of anti-alpha(v)beta3 antibodies resulted in a modest down-regulation of MMP-2. Our results indicate that the prese...Continue Reading

Citations

Aug 21, 2010·Cell Biology International·Marzanna Cechowska-PaskoEdward Bańkowski
Mar 7, 2003·American Journal of Physiology. Renal Physiology·Paraskevi V KitsiouEffie C Tsilibary
May 1, 2021·Cancers·Colin H QuinnElizabeth A Beierle
Sep 6, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Teresa RobledoEduardo Pérez Salazar

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