Effects of collagen prosthesis cross-linking on long-term tissue regeneration following the repair of an abdominal wall defect

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Gemma PascualJuan M Bellón

Abstract

Collagen prostheses used to repair abdominal wall defects, depending on their pretreatment (noncross-linked vs. cross-linked), besides repair may also achieve tissue regeneration. We assessed the host tissue incorporation of different bioprostheses using a new tool that combines immunofluorescence confocal microscopy with differential interference contrast images, making it possible to distinguish newly formed collagen. Partial hernial defects in the abdominal wall of rabbits were repaired using cross-linked/noncross-linked bioprostheses. Expanded polytetrafluoroethylene (ePTFE) was used as control. After 14/30/90/180 days of implant, specimens were taken for microscopy, immunohistochemistry, and quantitative-reverse transcription-polymerase chain reaction to determine host tissue ingrowth and collagen I/III protein and 1a1/3a1 gene expression. Shrinkage and stress resistance were also examined. At 14 days, cross-linked prostheses had suffered significantly less shrinkage than ePTFE or noncross-linked prostheses. Significantly higher shrinkage was recorded for ePTFE in the longer term. Microscopy revealed encapsulation of ePTFE by neoformed tissue, while the bioprostheses became gradually infiltrated by host tissue. Noncross-li...Continue Reading

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Citations

Dec 15, 2015·Surgical Infections·Bárbara Pérez-KöhlerJuan Manuel Bellón
Nov 21, 2012·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·N J SmartJ A Hunt
Jun 5, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·G PascualJ M Bellón
Aug 8, 2012·International Journal of Surgery·Neil J SmartSamuel Marquez
Dec 18, 2014·Tissue Engineering. Part B, Reviews·Luis M DelgadoDimitrios I Zeugolis
Jun 14, 2016·Scientifica·Gamal KarroufKhaled Abouelnasr

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