Effects of combination therapy using basic fibroblast growth factor and mature adipocyte-derived dedifferentiated fat (DFAT) cells on skin graft revascularisation

Journal of Plastic Surgery and Hand Surgery
Takashi AsamiHiroaki Nakazawa

Abstract

Although the benefits of basic fibroblast growth factor (bFGF) for wound healing and angiogenesis are well known, its effects on the process of skin graft revascularisation have not been clarified. It was hypothesised that bFGF would be beneficial to promote taking of skin grafts, but that the effect might be limited in the case of bFGF monotherapy. Therefore, this study investigated the efficacy of combination therapy using bFGF and dedifferentiated fat (DFAT) cells. DFAT cells have multilineage differentiation potential, including into endothelial cells, similar to the case of mesenchymal stem cells (MSC). Commercially available human recombinant bFGF was used. DFAT cells were prepared from SD strain rats as an adipocyte progenitor cell line from mature adipocytes. Full-thickness skin was lifted from the back of SD strain rats and then grafted back to the original wound site. Four groups were established prior to skin grafting: control group (skin graft alone), bFGF group (treated with bFGF), DFAT group (treated with DFAT cells), and combination group (treated with both bFGF and DFAT cells). Tissue specimens for histological examination were harvested 48 hours after grafting. The histological findings for the bFGF group showe...Continue Reading

References

Dec 1, 1988·Trends in Pharmacological Sciences·L Schweigerer
May 1, 1969·Plastic and Reconstructive Surgery·J M ConverseD L Ballantyne
Jun 1, 1994·Annals of Surgery·D M CooperM C Robson
Feb 1, 1996·Burns : Journal of the International Society for Burn Injuries·H J WangD M Chang
May 16, 1998·Plastic and Reconstructive Surgery·K SoejimaK Sasaki
Jan 10, 2001·Plastic and Reconstructive Surgery·M MaedaH Yamamoto
Sep 11, 2004·Biochemical and Biophysical Research Communications·Ken YagiKoichiro Kano
Oct 4, 2005·Burns : Journal of the International Society for Burn Injuries·Sadanori AkitaAkiyoshi Hirano
Mar 11, 2006·Burns : Journal of the International Society for Burn Injuries·Kazutaka SoejimaMasaki Takeuchi
Dec 8, 2007·Journal of Cellular Physiology·Taro MatsumotoHideo Mugishima
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stephan BarrientosMarjana Tomic-Canic
Aug 19, 2009·Journal of Molecular and Cellular Cardiology·Medet JumabayNoboru Fukuda
Feb 4, 2010·Plastic and Reconstructive Surgery·Summer E HansonPeiman Hematti
Nov 18, 2010·Methods in Molecular Biology·Hiroshi Mizuno, Masaki Nambu
Jul 2, 2011·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·Maximilian AckermannMoritz A Konerding
Jul 26, 2011·Microvascular Research·Maurizio CalcagniNicole Lindenblatt
Sep 25, 2012·Journal of Molecular and Cellular Cardiology·Medet JumabayKristina I Boström

❮ Previous
Next ❯

Citations

Jun 10, 2020·Scientific Reports·Hirofumi WatanabeTaro Matsumoto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.