Effects of combined 17beta-estradiol with TCDD on secretion of chemokine IL-8 and expression of its receptor CXCR1 in endometriotic focus-associated cells in co-culture

Human Reproduction
Ying-Li ShiDa-Jin Li

Abstract

Chemokines play an important role in the pathogenesis of endometriosis. In the present study, the transcription of 18 chemokine receptors in eutopic endometrium and ectopic tissue with endometriosis was first analysed by RT-PCR. Dioxin, an air pollutant, and estrogen are reported to be associated with endometriosis. The regulatory mechanisms of dioxin and estrogen in the expression of CXCR1/IL-8 in the corresponding cells will help in elucidating roles of the chemokine in the aetiology of endometriosis. CXCR1, a type of chemokine receptor, was over-expressed in endometriotic tissue. The high translation of the receptor and its ligand, interleukin (IL-8), in endometriotic tissue was then demonstrated by immunochemistry. Estradiol and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) alone inhibited expression of CXCR1, whereas the combination of estradiol with TCDD up-regulated the expression. TCDD promoted IL-8 secretion by human pelvic mesothelial cells (HPMC), and 17beta-estradiol magnified the stimulatory effect. Both 17beta-estradiol and TCDD alone inhibited IL-8 secretion of U937 (a cell line of monocyte), but combination of 17beta-estradiol and TCDD had no further inhibitory effect. The co-culture of endometrial stromal cells (E...Continue Reading

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Citations

Mar 30, 2013·Human Reproduction Update·Fernando M ReisRobert N Taylor
Sep 12, 2015·BioMed Research International·Jie ChenYing-Li Shi
Jan 26, 2017·American Journal of Reproductive Immunology : AJRI·Justyna SikoraZdzisława Kondera-Anasz
Nov 15, 2019·Scientific Reports·Tamara KnificTea Lanišnik Rižner
Feb 13, 2014·Experimental and Therapeutic Medicine·Dong-Ping YuanYing-Li Shi
Sep 15, 2020·Hormone Molecular Biology and Clinical Investigation·Konstantinos NirgianakisMichael D Mueller
Aug 3, 2021·Frontiers in Cellular and Infection Microbiology·Clarissa Leal Silva E SouzaLucas Miranda Marques

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