PMID: 7521738Jan 1, 1993Paper

Effects of combined administration of FK 506 and the purine biosynthesis inhibitors mizoribine or mycophenolic acid on lymphocyte DNA synthesis and T cell activation molecule expression in human mixed lymphocyte cultures

Transplant Immunology
A W ThomsonA Zeevi

Abstract

Our objective was to obtain new information on the in vitro antilymphocytic action of the cytokine synthesis inhibitor FK 506 and the purine biosynthesis inhibitors mycophenolic acid (MPA; the active moiety of RS61443) and mizoribine (MZB) when used alone or in combination. When added at the initiation of six-day human mixed lymphocyte cultures (MLC), FK 506, MPA or MZB exhibited dose-dependent inhibition of T-lymphocyte DNA synthesis. FK 506, however, was 100-fold more potent than MPA, and 10,000-fold more potent than MZB. Combination of FK 506 with either MPA or MZB, each at suboptional concentrations, produced no more than additive inhibitory effects on 3H thymidine incorporation. Two-colour flow cytometric analysis of lymphocytes revealed that none of the drugs affected cell surface activation molecule expression (CD25 = IL-2R 55 kD alpha-chain, HLA-DR or CD71 = transferrin receptor [TR]) on allostimulated CD4+ or CD8+ cells harvested at three days of culture. By day six, however, all three agents, at levels which markedly inhibited proliferation, suppressed the expression of activation markers on both CD4+ and CD8+ cells. Also at day six, inhibition of activation molecule expression on CD4+ cells was achieved with the comb...Continue Reading

References

Feb 1, 1992·Transplantation·H W SollingerR S Kauffman
Apr 1, 1992·Immunology Today·S L Schreiber, G R Crabtree
Jan 1, 1992·Autoimmunity·A W Thomson, T E Starzl
Jul 1, 1991·Transplantation·J M ArmitageS Todo
Jan 1, 1991·Transplantation·K P PlatzA C Allison
Aug 23, 1990·Nature·J DownwardD A Cantrell
Oct 28, 1989·Lancet·T E StarzlA Jain

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Citations

Dec 1, 1996·Journal of Clinical Pharmacology·S E Hughes, S A Gruber
Dec 9, 2003·Journal of Immunological Methods·Markus J BartenJan F Gummert
Jul 6, 2000·Immunopharmacology·A C Allison, E M Eugui
Sep 12, 2003·Transplant Immunology·Masanori Abe, Angus W Thomson
Oct 26, 2002·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Guenter WeigelMathias M Mueller
Feb 24, 2001·Transplantation·K Izeradjene, J P Revillard
Sep 15, 2015·Expert Review of Clinical Immunology·Chi Chiu Mok
Mar 10, 2001·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Y Yashima, T Ohgane
Sep 24, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Markus J BartenRandall E Morris
Mar 10, 2001·American Journal of Public Health·UNKNOWN American Public Health Association
Mar 10, 2001·American Journal of Public Health·UNKNOWN American Public Health Association
Mar 17, 2001·American Journal of Public Health·UNKNOWN American Public Health Association
Mar 10, 2001·American Journal of Public Health·UNKNOWN American Public Health Association
Feb 13, 2014·Basic & Clinical Pharmacology & Toxicology·Katharine H Abbott-Banner, Clive P Page
Jan 29, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·P C BaerH Geiger
Jun 11, 2009·British Journal of Pharmacology·Katharine H Banner, Neil J Press
Aug 18, 2020·European Journal of Clinical Pharmacology·Rui DaiPan Chen
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A MehlingS Beissert
Aug 30, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Chi Chiu Mok, Kar Neng Lai

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