Oct 1, 1980

Effects of combined glutamine and serum deprivation on glucose control of hexose transport in mammalian fibroblast cultures

Proceedings of the National Academy of Sciences of the United States of America
H M KalckarR A Laursen

Abstract

Regulation of hexose transport in NIL hamster fibroblasts has been studied in confluent cultures preconditioned for 24 hr in media deprived of glutamine or of serum or of both. Cultures maintained in media containing dialyzed fetal calf serum and 4 mM glutamine accumulated up to 72 nmol of glutamine per mg of cell protein; in contrast, cells deprived of glutamine contained less than 1 nmol/mg of cell protein. Glutamine elicited a general enhancement of hexose transport compared with transport in glutamine-deprived cultures. This enhancement was particularly pronounced in glucose-fed cultures which in the absence of glutamine showed conspicuously low transport activity. When maintained in glucose media, cultures deprived of serum also showed a marked loss of hexose transport which, in this case, was not compensated for by addition of glutamine. However, regardless of the presence or absence of glutamine, these cultures were able to develop the usual transport enhancement response to glucose starvation. Moreover, 2,4-dinitrophenol was also able to elicit a pronounced enhancement of hexose transport in the glucose-fed cultures; this effect surpassed even the transport derepression observed in the glucose-starved cultures. In polyo...Continue Reading

  • References16
  • Citations12

References

  • References16
  • Citations12

Citations

Mentioned in this Paper

Structure of Calf of Leg
Hexose Transport
Specimen Type - Fibroblasts
Hexoses
2,4-Dinitrophenol
Cattle calf (organism)
Bos taurus
Hamsters
Protein Degradation, Metabolic
Glucose, (beta-D)-Isomer

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