Effects of combined ketamine/xylazine anesthesia on light induced retinal degeneration in rats.

PloS One
Blanca Arango-GonzalezFlorian Gekeler

Abstract

To explore the effect of ketamine-xylazine anesthesia on light-induced retinal degeneration in rats. Rats were anesthetized with ketamine and xylazine (100 and 5 mg, respectively) for 1 h, followed by a recovery phase of 2 h before exposure to 16,000 lux of environmental illumination for 2 h. Functional assessment by electroretinography (ERG) and morphological assessment by in vivo imaging (optical coherence tomography), histology (hematoxylin/eosin staining, TUNEL assay) and immunohistochemistry (GFAP and rhodopsin staining) were performed at baseline (ERG), 36 h, 7 d and 14 d post-treatment. Non-anesthetized animals treated with light damage served as controls. Ketamine-xylazine pre-treatment preserved retinal function and protected against light-induced retinal degeneration. In vivo retinal imaging demonstrated a significant increase of outer nuclear layer (ONL) thickness in the non-anesthetized group at 36 h (p<0.01) and significant reduction one week (p<0.01) after light damage. In contrast, ketamine-xylazine pre-treated animals showed no significant alteration of total retinal or ONL thickness at either time point (p>0.05), indicating a stabilizing and/or protective effect with regard to phototoxicity. Histology confirmed...Continue Reading

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Citations

Mar 18, 2017·The Journal of Veterinary Medical Science·Wei LiGuangneng Peng
Nov 20, 2018·Investigative Ophthalmology & Visual Science·Michael T MassengillAlfred S Lewin
Aug 5, 2017·Biological & Pharmaceutical Bulletin·Maho NakamuraHideaki Hara

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