Effects of combretastatin A-4 phosphate on canine normal and tumor tissue-derived endothelial cells

Research in Veterinary Science
Yusuke IzumiSatoshi Takagi

Abstract

Combretastatin A-4 phosphate (CA4P) selectively blocks tumor blood flow. However, the detailed mechanisms through which CA4P specifically affects tumor blood vessels are not well understood. Recent reports revealed that tumor tissue-derived endothelial cells (TECs) have various specific features in comparison with normal tissue-derived endothelial cells (NECs). Thus, abnormalities in TECs may be involved in the selective vascular blockade mechanism of CA4P. In this study, we evaluated the effects of CA4P on canine NECs and TECs using confocal microscopy. NECs exhibited different susceptibilities at subconfluence and at 100% confluence. In addition, inhibition of vascular endothelial cadherin (VE-cadherin) in NECs increased the sensitivity of the cells to CA4P. TECs seemed to be more susceptible to CA4P than NECs. The expression pattern of VE-cadherin in TECs was abnormal compared with that of NECs, suggesting that VE-cadherin may have functional abnormalities in these cells. Taken together, these results indicate that the tumor-vascular selectivity of CA4P may be related to VE-cadherin dysfunction in TECs.

References

May 1, 1990·The Journal of Cell Biology·R L HeimarkS M Schwartz
May 1, 1995·Journal of Comparative Pathology·L FerrerM Vilafranca
Apr 11, 2002·Nature Cell Biology·Colin Jamora, Elaine Fuchs
Nov 19, 2004·Cancer Research·Kyoko HidaMichael Klagsbrun
Jun 2, 2005·Nature Reviews. Cancer·Gillian M TozerBruce C Baguley
Apr 25, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Beth A SalmonDietmar W Siemann
Mar 13, 2009·Nature Reviews. Cancer·Johanna A Joyce, Jeffrey W Pollard
Mar 23, 2010·Biochemical and Biophysical Research Communications·Kohei MatsudaKyoko Hida
Mar 7, 2012·Cold Spring Harbor Perspectives in Medicine·Andrew C Dudley
Dec 9, 2014·The Journal of Veterinary Medical Science·Yusuke IzumiMasahiro Okumura
Nov 15, 2016·European Journal of Pharmacology·Zhexuan LinWenhong Luo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Bulletin du cancer
P Verdier-PinardC Bailly
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Thomas NielsenLeif Østergaard
Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
Susan M GalbraithG J S Rustin
Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
Helen AndersonPatricia M Price
© 2022 Meta ULC. All rights reserved