Effects of concentrated growth factors on proliferation and osteogenic differentiation in Beagle adipose-derived stem cells

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Xiangyu MaYong Li

Abstract

To investigate the effect of concentrated growth factor (CGF) on proliferation and differentiation in Beagle adipose-derived stem cells (ADSCs).
 Methods: ADSCs were isolated from adipose tissue of healthy Beagles and cultured. The multi-directional differentiation potential of ADSCs was identified. The ADSCs were assigned to a CGF group and a control group. The rate of proliferation was analyzed by CCK-8 assay. The osteogenic differentiation capability was detected by ALP staining after the osteoinduction. Bone formation-related gene expression was detected by RT-PCR.
 Results: CGF promoted the proliferation of ADSCs in vitro. ADSCs in the CGF group showed higher level of ALP activity than that in the control group (P<0.05). CGF stimulated the expression of the genes associated with osteogenesis, such as Col-I and Runx2. 
 Conclusion: CGF can promote the proliferation and osteogenic differentiation in ADSCs in vitro.

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Related Papers

Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery
Guang-ping ChenXiang Xu
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Yan LiZhendong Liu
© 2022 Meta ULC. All rights reserved