Effects of continuous calcitonin treatment on osteoclast-like cell development and calcitonin receptor expression in mouse bone marrow cultures

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
M IkegameD M Findlay

Abstract

Continuous treatment with calcitonin (CT) to inhibit osteoclastic bone resorption results in acquired resistance. The mechanisms of this "escape" phenomenon are not yet established. The aim of this study was to examine the effects of continuous treatment with CT on the generation of osteoclasts and calcitonin receptor (CTR) expression in mouse bone marrow cultures. This was done by daily CT treatment of mouse bone marrow cultures from day 0, when only undifferentiated mononuclear precursors of osteoclast-like cells were present, or commencing from day 6, when differentiated osteoclast-like cells were abundant. The response to CT treatment was determined by quantitation of cells positive for tartrate-resistant acid phosphatase (TRAP) and binding of 125I-salmon CT. Calcitonin receptor and TRAP mRNA levels were determined using semi-quantitative reverse transcription/polymerase chain reaction. When cultures were treated with CT from day 0, TRAP-positive multinucleated cells appeared. These cells expressed only very low levels of CTR or CTR mRNA and were morphologically indistinguishable from osteoclast-like cells formed in control cultures. They also displayed the ability to resorb bone. Continuous CT treatment of cultures from da...Continue Reading

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Citations

Jun 5, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M RousseauM Fouchereau-Peron
Dec 13, 2018·Physiological Reviews·Dorit NaotJillian Cornish
Apr 6, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A LuchinM C Ostrowski
Mar 4, 2005·Treatments in Endocrinology·Manuel Muñoz-TorresMezquita Pedro Raya
Sep 24, 2020·International Journal of Endocrinology·Jingbo XieQingshan Sun
Aug 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J M QuinnT J Martin
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Paul H AndersonHoward A Morris

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