PMID: 8610519Jan 1, 1995Paper

Effects of contrast media on renal epithelial cells in culture

Acta Radiologica. Supplementum
K J AndersenE I Christensen

Abstract

Proximal and distal tubular cells in culture have been exposed to various roentgen contrast media (CM) at concentrations of 0 to 100 mg I/ml for 22 hours to study cellular mechanisms that may be involved in CM-induced nephropathy. The effects on cell morphology were assessed by electron microscopy and cell viability was evaluated. Levels of brush border and lysosomal marker enzymes in the culture medium were assayed biochemically. Morphological examination showed that CM induced a concentration-dependent formation of large cytoplasmic vacuoles in both cell lines. Cellular damage was observed more frequently after exposure to low-osmolal rather than the iso-osmolal CM iodixanol; the low-osmolal CM causing more cell death and inhibiting cellular growth to a greater degree than did iodixanol. In cultures of both cell lines the CM produced a concentration-dependent increase in brush border marker enzyme activity. While an increase in lysosomal enzyme activity was seen at low concentrations, a decrease in activity occurred at high concentrations. Earlier investigations have demonstrated that the nonionic CM have less pronounced effects on the cell lines studied than ionic CM. The results presented here indicate that the effects of t...Continue Reading

References

Jul 1, 1991·AJR. American Journal of Roentgenology·E M LautinS Sprayregen
Jul 1, 1991·AJR. American Journal of Roentgenology·M A Bettmann
Sep 1, 1989·Investigative Radiology·A NordbyO A Haugen
Dec 1, 1985·Journal of Cellular Physiology·A WohlwendL Orci
Jan 1, 1970·The Journal of Pediatrics·A B GruskinC M Edelmann
Jan 1, 1980·The American Journal of Physiology·L J MandelM M Grafton
Nov 1, 1994·Investigative Radiology·K J AndersenH Vik
Jan 1, 1993·Contributions to Nephrology·K J Andersen, H Vik

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Citations

Jan 1, 1995·Acta Radiologica. Supplementum·M DobrotaH Vik
Jan 3, 2001·American Journal of Physiology. Renal Physiology·K HardiekC Deitrick
May 19, 2019·The Journal of Pharmacology and Experimental Therapeutics·Dakota B Ward, Monica A Valentovic
Aug 24, 2019·International Journal of Molecular Sciences·Dakota B WardMonica A Valentovic
Dec 25, 2003·Journal of Virology·Christopher B BuckJohn T Schiller

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