Effects of controlled dual growth factor delivery on bone regeneration following composite bone-muscle injury

Acta Biomaterialia
Ramesh SubbiahRobert E Guldberg

Abstract

The objective of this study was to investigate the controlled release of two growth factors (BMP-2 and VEGF) as a treatment strategy for bone healing in clinically challenging composite injuries, consisting of a femoral segmental bone defect and volumetric muscle loss. This is the first investigation of dual growth factor delivery in a composite injury model using an injectable delivery system consisting of heparin microparticles and alginate gel. The loading efficiency of growth factors into these biomaterials was found to be >90%, revealing a strong affinity of VEGF and BMP-2 to heparin and alginate. The system could achieve simultaneous or tunable release of VEGF and BMP-2 by varying the loading strategy. Single growth factor delivery (VEGF or BMP-2 alone) significantly enhanced vascular growth in vitro. However, no synergistic effect was observed for dual growth factor (BMP-2 + VEGF) delivery in vitro. Effective bone healing was achieved in all treatment groups (BMP-2, simultaneous or tunable delivery of BMP-2 and VEGF) in the composite injury model. The mechanics of the regenerated bone reached a maximum strength of ~52% of intact bone with tunable delivery of VEGF and BMP-2. Overall, simultaneous or tunable co-delivery of...Continue Reading

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Citations

Jan 13, 2021·Biomaterials Science·Jonathan DoroginMarian H Hettiaratchi
Jan 13, 2021·Journal of Tissue Engineering and Regenerative Medicine·Ramesh SubbiahLuiz E Bertassoni
Nov 16, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ramesh SubbiahLuiz E Bertassoni

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