Effects of controlled-frequency moderate electric fields on pectin methylesterase and polygalacturonase activities in tomato homogenate

Food Chemistry
Chaminda P Samaranayake, Sudhir K Sastry

Abstract

The effect of controlled-frequency moderate electric field treatments on pectin methylesterase and polygalcturonase activities in tomato homogenate was investigated by subjecting identically treated control and electrically-treated samples to the same temperature history. Additionally, a model was developed for the motion of the enzyme molecules subjected to an electric field. Results show that the application of electric fields at a low field strength (0.4V/cm) constant temperature (65°C) has a statistically significant effect on pectin methylesterase activity, typically at or lower than 60 Hz. At higher frequencies, the effects are negligible. Molecular motion simulations suggest that the efficacy at low frequencies may be due to the amplitude of motion being of the order of the intermolecular distance for water. Higher frequencies result in small overall displacements due to rapid reversals in the direction of motion.

References

Feb 26, 2000·Journal of Agricultural and Food Chemistry·I Van Den BroeckM E Hendrickx
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·L FedericiD Tsernoglou
Apr 12, 2002·FEBS Letters·Kenth JohanssonHans Eklund
Oct 3, 2002·Journal of Agricultural and Food Chemistry·Gordon E AnthonDiane M Barrett
Mar 17, 2005·Journal of Agricultural and Food Chemistry·Netsanet Shiferaw TerefeMarc Hendrickx
Jan 5, 2006·Journal of Agricultural and Food Chemistry·Gordon E Anthon, Diane M Barrett
Oct 5, 2006·Journal of Chromatography. a·Irena Vovk, Breda Simonovska
May 3, 2007·The Journal of Physical Chemistry. B·Akin BudiIrene Yarovsky
Sep 22, 2011·Journal of Agricultural and Food Chemistry·Ricardo N PereiraAntónio A Vicente
Nov 8, 2014·Journal of the Science of Food and Agriculture·Matthew T HeldDiane M Barrett

❮ Previous
Next ❯

Citations

May 6, 2016·Journal of Food Science·Sudhir K Sastry
Jan 26, 2017·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Diederich Aguilar-MachadoJulio Montañez
Jan 6, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Danmei HuangYi Qin
Aug 5, 2019·Bioelectrochemistry·Felix SchottroffHenry Jaeger
Nov 23, 2020·Enzyme and Microbial Technology·Emily K Durham, Sudhir K Sastry
Jun 29, 2021·Physical Chemistry Chemical Physics : PCCP·Chaminda P Samaranayake, Sudhir K Sastry
Nov 26, 2020·Food Research International·Rui M RodriguesAntónio A Vicente

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.