Effects of controlled released BMP-7 on markers of inflammation and degradation during the cultivation of human osteoarthritic chondrocytes

Journal of Biomaterials Applications
K GavenisB Schmidt-Rohlfing

Abstract

The aim of the present study is to investigate the effects of BMP-7 released from polylactide microspheres on the appearance of various catabolic and inflammatory cytokines secreted by osteoarthritic chondrocytes cultivated in a collagen gel. Articular chondrocytes of 15 patients suffering from osteoarthritis are transferred to a collagen type-I gel. Additionally, BMP-7 encapsulated into polylactide microspheres (50 ng BMP-7/mL gel) is added. After 14 days, gene expression and protein appearance of various genes involved in matrix turnover and inflammation are investigated by immunohistochemical staining and RT-PCR and compared to untreated controls. TNF-α, MMP-13, IL-6, IL-1β, and VEGF gene expressions are decreased in the treatment group. In contrast, BMP-7-induced matrix synthesis is not affected, leaving collagen type-II (Col-II) gene expression to be elevated, while collagen type-I (Col-I) is decreased. In summary, controlled release of low concentrated BMP-7 from polylactide microspheres leads to a decrease in gene expression of the investigated inflammation and matrix degradation markers whereas matrix synthesis is induced.

References

Aug 1, 1997·Arthritis and Rheumatism·O LindyC López-Otín
May 27, 1999·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·H E KoeppG A Homandberg
Nov 9, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·C R FlanneryS A Jones
Oct 18, 2001·Clinical Orthopaedics and Related Research·M Lotz
Jan 12, 2002·Kidney International·Stephen E GouldHaimanti Dorai
Aug 8, 2002·Biomaterials·Yasuhiko IwasakiHai Bang Lee
Aug 23, 2002·Current Opinion in Rheumatology·Thomas AignerLinda Sandell
Aug 28, 2002·Scandinavian Journal of Rheumatology·Bernhard Schmidt-RohlfingUlrich Schneider
Jan 1, 2003·The American Journal of Pathology·Hiroyuki EnomotoYasunori Okada
Aug 9, 2003·Arthritis and Rheumatism·L HaywoodD A Walsh
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·Charis MerrihewSusan Chubinskaya
Aug 19, 2004·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J ShiR G Dullea
Oct 14, 2004·Clinical Orthopaedics and Related Research·Steven R Goldring, Mary B Goldring
Sep 27, 2005·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Christopher B ForsythRichard F Loeser
Dec 22, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Karsten GavénisBernhard Schmidt-Rohlfing
Jan 6, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Susan ChubinskayaDavid C Rueger
Sep 6, 2007·Journal of Cellular Physiology·Mary B Goldring, Steven R Goldring
Jan 19, 2008·The International Journal of Artificial Organs·R Muller-RathU Schneider
May 7, 2008·Current Rheumatology Reports·Tim Hardingham
Aug 9, 2008·The Journal of Knee Surgery·Susan ChubinskayaBrian J Cole

❮ Previous
Next ❯

Citations

Mar 21, 2012·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Sven NebelungBjörn Rath
Jul 8, 2011·PM & R : the Journal of Injury, Function, and Rehabilitation·Michael B Mueller, Rocky S Tuan
Sep 26, 2020·Nature Communications·Maria Angelica CortezJames W Welsh
May 4, 2021·Frontiers in Cell and Developmental Biology·Jin ChengYingfang Ao
May 1, 2021·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Zubeyir ElmazogluÇimen Karasu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay

Software Mentioned

Discus
SAS

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.