Effects of cooling and warming rates during vitrification on fertilization of in vitro-matured bovine oocytes

Cryobiology
S HochiA Hanada

Abstract

The purpose of this study was to clarify the relationship of cooling rates (CR) and warming rates (WR) during vitrification with postwarming viability of in vitro-matured bovine oocytes. In Experiment 1, oocytes were vitrified in a solution containing 7.2 M ethylene glycol and 1.0 M sucrose by use of open-pulled glass capillaries with five different outer diameters and were warmed by placement of the capillaries into 0.25 M sucrose solution. The capillaries of 2000-, 1400-, 1000-, 630-, and 440-mm diameters provided CR of 2000, 3000, 5000, 8000, and 12,000 degrees C/min and WR of 5000, 8000, 17,000, 33,000, and 62,000 degrees C/min, respectively. In oocytes vitrified in capillaries of 1400-mm diameter (CR, 3000 degrees C/min; WR, 8000 degrees C/min), the morphological survival rate (86% of vitrified), penetration rate (79% of inseminated), and normal fertilization rate (69% of penetrated) were higher or tended to be higher than those in the other vitrification groups. In Experiment 2, oocytes cooled at 2000, 3000, or 12,000 degrees C/min were warmed at 8000 degrees C/min, and oocytes cooled at 3000 degrees C/min were warmed at 5000, 8000, or 33,000 degrees C/min. Among these CR-WR combinations, cooling of oocytes at 3000 degree...Continue Reading

Citations

Oct 1, 2011·Nanomedicine·Xiaohui ZhangUtkan Demirci
Jan 30, 2004·Reproductive Biomedicine Online·Juergen LiebermannMichael J Tucker
Feb 10, 2007·Molecular Reproduction and Development·S SuccuS Naitana
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Mar 3, 2012·Clinical and Experimental Reproductive Medicine·Mi Ra ShinChun Kyu Lim
Sep 5, 2020·Zygote : the Biology of Gametes and Early Embryos·Linda DujíčkováFrantišek Strejček
Nov 26, 2016·Reproduction in Domestic Animals = Zuchthygiene·L Fernandez-Gonzalez, K Jewgenow
Oct 6, 2017·Biopreservation and Biobanking·Guobo QuanQionghua Hong
Mar 30, 2010·Reproduction in Domestic Animals = Zuchthygiene·C YamadaJ A Visintin
Jan 1, 2018·Reproduction, Fertility, and Development·Gary D Smith, Shuichi Takayama
Feb 10, 2011·Human Reproduction·Javier I GarcíaLuis Noriega-Hoces
Mar 12, 2021·ACS Applied Materials & Interfaces·Yu XiaShi-Shang Guo

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