PMID: 16529319Mar 15, 2006Paper

Effects of core binding factor alpha1 on promotion of osteoblastic differentiation from marrow mesenchymal stem cells

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Huazhuang LiGuoning Guo

Abstract

To observe effects of the core binding factor alpha1 (Cbfalpha1) in its promoting differentiation of the rabbit marrow mesenchymal stem cells (MSCs) into osteoblasts. The rabbit marrow MSCs were isolated and cultured in vitro and were divided into 3 groups. In the control group, the marrow MSCs were cultured by DMEM; in the single inducement group, they were cultured by the condition medium (DMEM, 10% fetal bovine serum, dexamethasone 10 mmol/L, vitamin C 50 mg/L, and beta-GP 10 mmol/L); and in the experimental group, they were transfected with AdEasyl/Cbfalpha1, and then were cultured by the condition medium. The alkaline phosphatase (ALP) activity and the expression of osteocalcin as the osteoblast markers were measured with the chemohistological and immunohistochemical methods at 3 days, 1, 2, 3, and 4 weeks after inducement. More than 90% MSCs were grown well in vitro. The GFP was positive in MSCs after their being transfected with AdEasyl/Cbfalpha1. The ALP activity and the expression of osteocalcin were significantly upregulated in the transfection group compared with those in the single inducement group and the control group at 1, 2, 3, and 4 weeks (P < 0.05). The mineralized node began to appear at 2 weeks in the experi...Continue Reading

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