Effects of Corneal Hydration on Brillouin Microscopy In Vivo

Investigative Ophthalmology & Visual Science
Peng ShaoSeok-Hyun Yun

Abstract

To investigate how corneal hydration affects the Brillouin frequency of corneal stroma. From a simple analytical model considering the volume fraction of water in corneal stroma, we derived the dependence of Brillouin frequency on hydration and hydration-induced corneal thickness variation. The Brillouin frequencies of fresh ex vivo porcine corneas were measured as their hydration was varied in dextran solution and water. Healthy volunteers (8 eyes) were scanned in vivo repeatedly over the course of 9 hours, and the diurnal variations of Brillouin frequency and central corneal thickness (CCT) were measured. The measured dependence of Brillouin frequency on hydration, both ex vivo and in vivo, agreed well with the theoretical prediction. The Brillouin frequencies of human corneas scanned immediately after waking were on average ∼25 MHz lower than their daytime average values. For stabilized corneas, the typical variation of Brillouin frequency was ± 7.2 MHz. With respect to CCT increase or swelling, the Brillouin frequency decreased with a slope of -1.06 MHz/μm in vivo. The ex vivo and in vivo data agree with our theoretical model and support that the effect of corneal hydration on Brillouin frequency comes predominantly from th...Continue Reading

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Citations

Sep 25, 2019·Nature Methods·Robert PrevedelGiuseppe Antonacci
Jan 3, 2019·Investigative Ophthalmology & Visual Science·Brecken J BlackburnAndrew M Rollins
Sep 3, 2019·Current Eye Research·Keyton ClaysonJun Liu
Jun 17, 2020·Investigative Ophthalmology & Visual Science·Sabine KlingFarhad Hafezi
Nov 14, 2019·Cellular and Molecular Bioengineering·Christina ConradGiuliano Scarcelli
Mar 21, 2019·Biomedical Optics Express·Antonio Fiore, Giuliano Scarcelli
Aug 25, 2020·Translational Vision Science & Technology·Vinicius S De StefanoWilliam J Dupps
Aug 25, 2020·Translational Vision Science & Technology·Joshua N WebbGiuliano Scarcelli
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Apr 3, 2019·Communications Biology·Raffaella MercatelliRiccardo Cicchi
May 16, 2019·Biomedical Optics Express·Miloš Nikolić, Giuliano Scarcelli
Feb 21, 2021·Experimental Eye Research·Jillian Chong, William J Dupps
Mar 11, 2021·Journal of Ophthalmology·Sang Beom HanJodhbir S Mehta
Jan 12, 2020·Communications Biology·Raffaella MercatelliRiccardo Cicchi
Apr 3, 2021·Biomedical Optics Express·Seungmi RyuSeok-Hyun Yun
Dec 15, 2020·Journal of Cataract and Refractive Surgery·Emilio A Torres-NettoSabine Kling
Jul 28, 2021·Translational Vision Science & Technology·Tanner J FergusonWilliam J Dupps
Aug 8, 2021·International Journal of Molecular Sciences·Rafael J Jiménez RioboóMaria Victoria Gómez-Gaviro

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