Effects of corticotropin-releasing hormone (CRH) on the synthesis and secretion of proopiomelanocortin-related peptides in the anterior pituitary: a study using CRH-deficient mice

Neuroscience Letters
Yoshiko FukudaToshihiro Suda

Abstract

Corticotropin-releasing hormone (CRH) mainly regulates the synthesis and secretion of adrenocorticotropin (ACTH) in the anterior pituitary (AP). By using CRH-deficient mice (CRH KO), we investigated the role of CRH in the processing of proopiomelanocortin (POMC), a precursor of ACTH, beta-lipotropic hormone, and beta-endorphin (EP). In the basal condition, the plasma ACTH level was similar in CRH KO and wild-type mice (WT), while its response to pain stress in CRH KO was smaller than that in WT. Immunoreactive (ir) beta-EP contents in the AP of CRH KO were not significantly different from those of WT. In order to determine the different molecule profile of POMC-related peptides between WT and CRH KO, ir beta-EP contents extracted from AP of WT and CRH KO were assayed by gel filtration chromatography. The gel filtration analyses revealed that a higher molecular weight form of ir beta-EP, putative POMC, was increased in CRH KO, but the beta-EP peak level was small and similar between two groups. These results suggest that CRH has little influence on the basal release of ACTH and prohormone convertase-2 processing enzyme. On the other hand, it is essential for ACTH secretion under stress conditions, and CRH would affect on the pro...Continue Reading

References

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Citations

May 8, 2015·Endocrine Journal·Kazunori KageyamaMakoto Daimon
Oct 23, 2008·Regulatory Peptides·Kazunori KageyamaToshihiro Suda
Jul 26, 2005·Peptides·Richard J Bodnar, Gad E Klein
May 18, 2016·Endocrine Reviews·Pieter StijnenJohn W M Creemers
Jan 1, 2008·Gene Regulation and Systems Biology·Masanori Yoshida
Mar 29, 2011·Stress : the International Journal on the Biology of Stress·Kazunori KageyamaToshihiro Suda

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