Effects of crocin on inflammatory activities in human fibroblast-like synoviocytes and collagen-induced arthritis in mice

Immunologic Research
Longjie LiChang Liu

Abstract

Rheumatoid arthritis (RA) is a systemic autoimmune disease, characterized by the irreversible joint destruction resulted from the attack of inflammatory cells to the joints. Recent studies demonstrated that crocin is able to alleviate arthritis and suppress inflammatory responses, implying crocin as a potential promising antiarthritic agent. In this study, we confirmed the effect of crocin on RA and revealed its underlying mechanism by measuring lipopolysaccharides (LPS)-stimulated cytokine production in presence or absence of crocin. The effect of crocin was also tested in vivo using a mouse model of collagen-induced arthritis (CIA). It was found that crocin significantly repressed the LPS-induced expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in human fibroblast-like synoviocytes (FLS). We tested the effect of crocin on nuclear factor-kappa B (NF-κB) signaling and observed that cells pre-treated with 500 μM of crocin exhibited lower levels of LPS-induced p-IκBα, p-IκB kinase (IKK) α/β, and p65 expression than those of untreated cells. In addition, we found when cells were stimulated with IKKβ, crocin pre-treatment showed significantly inhibitory effect on the luciferase activity of IL-1β. In vivo r...Continue Reading

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Citations

Aug 21, 2019·BioFactors·Shahla KoraniAmirhossein Sahebkar
Jan 31, 2020·Pharmacological Reports : PR·Mehrnaz MehrabaniAlireza Malayeri
Jul 9, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mahmoud HashemzaeiRamin Rezaee
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuguang GuanDe-An Guo

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Methods Mentioned

BETA
Assay
fluorescence microscopy
PCR
enzyme-linked immunosorbent assay
ELISA

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