Effects of cryopreservation and hypothermic storage on cell viability and enzyme activity in recombinant encapsulated cells overexpressing alpha-L-iduronidase

Artificial Organs
Fabiana Quoos MayerUrsula Matte

Abstract

Here, we show the effects of cryopreservation and hypothermic storage upon cell viability and enzyme release in alginate beads containing baby hamster kidney cells overexpressing alpha-L-iduronidase (IDUA), the enzyme deficient in mucopolysaccharidosis type I. In addition, we compared two different concentrations of alginate gel (1% and 1.5%) in respect to enzyme release from the beads and their shape and integrity. Our results indicate that in both alginate concentrations, the enzyme is released in lower amounts compared with nonencapsulated cells. Alginate 1% beads presented increased levels of IDUA release, although this group presented more deformities when compared with alginate 1.5% beads. Importantly, both encapsulated groups presented higher cell viability after long cryopreservation period and hypothermic storage. In addition, alginate 1.5% beads presented higher enzyme release after freezing protocols. Taken together, our findings suggest a benefic effect of alginate upon cell viability and functionality. These results may have important application for treatment of both genetic and nongenetic diseases using microencapsulation-based artificial organs.

References

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Citations

Mar 16, 2011·Artificial Organs·Paul S Malchesky
Apr 14, 2012·International Journal of Biomaterials·Jennifer MumawSteven L Stice
May 27, 2011·Journal of Inherited Metabolic Disease·Ursula MatteRoberto Giugliani
Feb 20, 2013·Journal of Microencapsulation·Valeska Lizzi LagranhaUrsula Matte
Jan 31, 2016·Stem Cells Translational Medicine·Stephen SwiokloChe J Connon
Aug 24, 2013·Macromolecular Bioscience·Maria BulwanMaria Nowakowska
Feb 6, 2017·Journal of Materials Science. Materials in Medicine·Valeska Lizzi LagranhaUrsula Matte

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