Effects of cryopreservation and phenylacetate on biological characters of adherent LAK cells from patients with hepatocellular carcinoma

World Journal of Gastroenterology : WJG
Ning ZhengZhao-You Tang

Abstract

To improve the preparation of adherent lymphokine-activated killer (A-LAK) cells and to study the effects of cryopreservation and phenylacetate (PA) on biological characters of A-LAK cells. A-LAK cells were obtained from peripheral blood mononuclear cells (PBMCs) of the patients with hepatocellular carcinoma (HCC) by using L-phenylalanine methyl ester (PME) to deplete immunosuppressive monocytes. Proliferative activity of SMMC7721 cell line after treatment with phenylacetate (PA) was observed. A-LAK cells were treated with the supernatant of SMMC7721 cells that had been pretreated with PA. The changes of proliferation, cytotoxicity and phenotype of A-LAK cells were investigated after cryopreservation. The expansion of A-LAK cells (96.79 +/- 69.10 folds on Day 14) was significantly higher than that of non-adherent LAK (NA-LAK) cells (22.77 +/- 13.20) as well as conventional LAK cells (4.64 +/- 0.91). PA significantly suppressed the growth of SMMC7721 cells, and the inhibitor ratio was 46%. The supernatant of cultured tumor cells intensively suppressed the proliferation and cytotoxicity of A-LAK cells, but the suppressive effect of the supernatant was previously decreased after treatment with PA. Impairments in proliferation and ...Continue Reading

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Citations

Apr 30, 2003·World Journal of Gastroenterology : WJG·Li-Wu LinXiao-Dong Lin
Jan 1, 2003·World Journal of Gastroenterology : WJG·Nan-Hong TangXiao-Zhong Wang
May 5, 2017·International Reviews of Immunology·Senthilkumar PreethySamuel J K Abraham
Jun 5, 2002·World Journal of Gastroenterology : WJG·Lun-Xiu Qin, Zhao-You Tang
Apr 30, 2003·World Journal of Gastroenterology : WJG·Shao-Shan WangQing-Hui Qi
Apr 17, 2016·Applied Microbiology and Biotechnology·Akbar KhalilpourAli Khademhosseini
Oct 16, 2002·World Journal of Gastroenterology : WJG·Zhao-Chong ZengGeorge Iliakis
Dec 7, 2011·Transfusion·Allison Hubel

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