Effects of cultivation conditions on the production of natamycin with Streptomyces gilvosporeus LK-196

Enzyme and Microbial Technology
Jingle LiangPeilin Cen

Abstract

Natamycin is a very attractive antifungal agent with wide applications in medical and food industries. In order to improve the productivity of natamycin, the effects of cultivation conditions were investigated with Streptomyces gilvosporeus LK-196 in the shake flasks and 30-L fermentors. The results showed that dissolved oxygen and shear force would affluence the biosynthesis of natamycin significantly. The high concentration of natamycin (2.03g/L) was achieved under the suitable culture conditions in the shake flask scale. Further investigations in 30-L fermentors showed that the optimal pH was controlled at 6.0 during the whole bioprocess, and the dissolved oxygen level should be more than 30% by adjusting the aeration and agitation rates for high production of natamycin. Under these optimal conditions the high concentration of natamycin (3.94g/L) was achieved with Str. gilvosporeus LK-196 in the 30-L fermentor. Finally, the high-level fermentation process was successfully scaled up to 1000-L fermentors and 18,000-L fermentors in the pilot plant.

Citations

Mar 7, 2013·Applied Microbiology and Biotechnology·Dahong WangQiang Shi
Jun 4, 2008·Journal of Industrial Microbiology & Biotechnology·Dasari V Krishna RaoAdibhatla Kali S Bhujanga Rao
Mar 8, 2013·World Journal of Microbiology & Biotechnology·Nan ZhangXunli Liu
Feb 18, 2014·Applied Biochemistry and Biotechnology·Min LiZhixia Ji
Oct 30, 2015·Applied Microbiology and Biotechnology·Jesús F AparicioJavier Santos-Aberturas
Oct 25, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Ling WangGuo-Ying Zhou
Nov 30, 2019·Applied Microbiology and Biotechnology·Dandan TangZhinan Xu
Jul 30, 2017·Applied Microbiology and Biotechnology·Dahong WangShaobin Gu
Jun 10, 2008·Journal of Agricultural and Food Chemistry·Guan-Qun ChenLian-Xiang Du

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