PMID: 6168467Jul 1, 1981Paper

Effects of cyclic AMP and fluoride on phosphorylation of ribosomal protein S6 and on protein synthesis in rabbit reticulocytes

European Journal of Biochemistry
G A FLoyd, J A Traugh

Abstract

The effects of N6,O2-dibutyryl-adenosine 3',5'-monophosphate (Bt2cAMP) and sodium fluoride on the phosphorylation of ribosomal proteins S6 and on protein synthesis were examined. Rabbit reticulocytes were incubated in a nutritional medium containing 32Pi in the presence and absence of Bt2cAMP (1mM) and 3-isobutyl-1-methyl-xanthine (1mM). In the control cells, four phosphorylated derivatives of S6 were observed, with most of the radioactivity in the monophosphorylated form. Upon addition of cyclic nucleotide, a twofold increase in the phosphorylation of ribosomal protein S6 was observed. This was accompanied by an increase of radioactive phosphate in the diphosphorylated derivative. No alteration in protein synthesis was observed upon addition of cAMP and analogues of cAMP in conjunction with 3-isobutyl-1-methyl-xanthine or theophylline. The effects of sodium fluoride on phosphorylation of S6 and on protein synthesis were examined also. At 5 mM sodium fluoride, protein synthesis was inhibited by 85%. A 2.5-fold increase in the phosphorylation of ribosomal protein S6 was observed with an accumulation of 32Pi in the diphosphorylated, triphosphorylated and tetraphosphorylated derivatives. Inhibition of protein synthesis coincided w...Continue Reading

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May 1, 1980·European Journal of Biochemistry·G A Floyd, J A Traugh

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Citations

Jan 1, 1985·The International Journal of Biochemistry·S Pickett, J C Lee
Aug 30, 1982·Biochimica Et Biophysica Acta·G ParadisG Lemieux
Jan 1, 1986·Molecular & General Genetics : MGG·F HerreraM Alvarado
Apr 29, 1987·Biochemical and Biophysical Research Communications·D TabariniO M Rosen

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