Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells.

Journal of Bacteriology
R V Gilden, R I Carp

Abstract

Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolished when cycloheximide was added up to 10 hr postinfection. In contrast, puromycin, another inhibitor of protein synthesis, did not suppress antigen production. The basis of this differential effect was the inability of puromycin to inhibit protein synthesis in the cells used. This was shown by the failure of the drug to depress the incorporation of labeled amino acid into protein and also failure to inhibit poliovirus synthesis. The puromycin preparation used was very effective in inhibiting poliovirus synthesis in HeLa cells. Thus, appearance of the SV40 T antigen is dependent on protein synthesis in infected cells.

References

Jul 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·R I CARPD KRITCHEVSKY
Dec 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·P H BLACKR J HUEBNER
Aug 1, 1964·The Journal of Experimental Medicine·J H POPE, W P ROWE
Aug 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·F RAPPJ L MELNICK
Mar 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R V GILDENV DEFEND

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Citations

Jan 15, 1971·International Journal of Cancer. Journal International Du Cancer·J AndersonJ J Trentin
Mar 13, 2015·Molecular Biology of the Cell·Idil UlenginTina H Lee
Aug 1, 1967·Journal of Virology·S KitT Kitahara

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