Effects of cyclooxygenase-2 inhibition on serum and tumor gastrins and expression of apoptosis-related proteins in colorectal cancer.

Digestive Diseases and Sciences
Peter C KonturekEckhart Hahn

Abstract

The objective of the present study was to determine the influence of cyclooxygenase-2 (COX-2) inhibition by Celecoxib (CLX) in humans with distal colorectal adenocarcinoma (CRC) on serum and tumor levels of progastrin and gastrin and serum levels of proinflammatory cytokines (IL-8, TNF-alpha). In addition, the effects of this CLX treatment on tumor and adjacent mucosa expression of gastrin, its receptors (CCK2), and COX-1 and COX-2, as well as protein expression of the active form of nuclear factor kappa B (NFkappa B) and the apoptotic-related proteins Bcl-2 and survivin, have been examined. Ten distal CRC patients were examined twice, once before and then after 14-day treatment with CLX (200 mg bid). Large biopsy samples were taken from the tumor and intact mucosa 10 cm above the tumor. For comparison, 20 age- and sex-matched healthy controls were enrolled and treated with CLX as CRC patients. Serum levels of IL-8 and TNF-alpha were measured by enzyme-linked immunosorbent assay, and serum levels of amidated gastrins and progastrin, by specific radioimmunoassay. The gene or protein expressions of progastrin, gastrin, CCK2, COX-1, COX-2, Bcl-2, and survivin as well as NFkappa B were determined by RT-PCR or Western blot in biopsy...Continue Reading

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Citations

Dec 14, 2012·World Journal of Gastroenterology : WJG·Eduardo ChuecaElena Piazuelo
Dec 12, 2012·Current Colorectal Cancer Reports·Pomila SinghCarrie Maxwell
Aug 23, 2011·Clinical Colorectal Cancer·Jonathan M HernandezDavid Shibata
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May 20, 2018·BMC Gastroenterology·Darina KohoutovaJan Bures
Aug 28, 2021·Biomedicines·Supattra ChaithongyotMichael Naumann

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