Effects of cyclophilin A on cell proliferation and gene expressions in human vascular smooth muscle cells and endothelial cells

The Journal of Surgical Research
Hui YangChangyi Chen

Abstract

Cyclophilin A (CypA) is a cytosolic protein which involves many biological functions including immune modulation, cell growth, tumorigenesis, and vascular disease. The objective of this study was to determine the effect of CypA on cell proliferation and several gene expressions in human endothelial cells and vascular smooth muscle cells. Human coronary artery endothelial cells (HCAEC), human lung microvascular endothelial cells (HMVEC-L), and human aorta smooth muscle cells (HAoSMC) were used in this study. Cells were treated with 10 nM CypA for 24 h. The cell proliferation was determined by [3H]thymidine incorporation. The mRNA levels of 13 genes including CD147 (receptor for CypA), PDGF-BB, endothelin-1 (ET-1), vascular endothelial growth factor receptor-1 (VEGFR-1), VEGFR-2, VEGFR-3, neuropilin-1 (NRP-1), NRP-2, eNOS, iNOS, nNOS, ICAM-1, and PECAM-1 were semiquantitatively determined by real time RT-PCR as standardized with a house keeping gene beta-actin. CypA significantly increased cell proliferation of HAoSMC and HMVEC-L by 31% and 45%, respectively, as compared to controls, but had no effect on HCAEC. Blocking CD147 did not affect the mitogenic action of CypA. In addition, CypA also significantly increased the mRNA expr...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Barbara SherryA Cerami
Sep 15, 1993·European Journal of Biochemistry·A Galat
Mar 3, 1997·The Journal of Experimental Medicine·A BillichP Peichl
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M L GagnonM Klagsbrun
Oct 31, 2000·Circulation Research·Z G JinB C Berk
Jun 8, 2001·The Journal of Biological Chemistry·S P LeeH Z Chae
Feb 14, 2002·Trends in Cardiovascular Medicine·Gera NeufeldYael Herzog
Apr 12, 2002·The Journal of Biological Chemistry·Vyacheslav YurchenkoMichael Bukrinsky

Citations

Feb 16, 2006·Immunology·Vyacheslav YurchenkoMichael Bukrinsky
Oct 10, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sumalee ObchoeiSopit Wongkham
Aug 7, 2007·Biochemical and Biophysical Research Communications·Huirong YangLong Yu
Apr 20, 2005·The Journal of Surgical Research·Wiley W Souba, David W McFadden
Oct 19, 2005·Circulation·Daniela TirziuMichael Simons
Jun 14, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Dominik G HaiderMehrdad Baghestanian
Aug 27, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Hong ChaiChangyi Chen
Apr 10, 2014·Biological Chemistry·Henrik Hoffmann, Cordelia Schiene-Fischer
Jan 11, 2011·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·R DavoliM F W te Pas
Dec 23, 2018·Free Radical Research·Ping-Fang ChiuChin-San Liu
May 30, 2006·Molecular Cancer Therapeutics·Lee M Ellis
Mar 1, 2021·International Immunopharmacology·Anastasiia KalininaLudmila Khromykh
Apr 16, 2021·Cancer Cell International·Amir Sadra ZangoueiMeysam Moghbeli

Related Concepts

Ascending Aorta Structure
Blood Capillaries
Cell Division Phases
Sinus Node Artery
Endothelium, Vascular
Muscle, Smooth, Vascular
Gene Expression
Cyclophilin A

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