PMID: 3764319Jan 1, 1986Paper

Effects of cyclosporine on human endothelial cell cultures

Toxicologic Pathology
A L Trifillis, M Hall-Craggs

Abstract

Reports of vascular changes in renal biopsies of transplant patients treated with cyclosporine prompted review of our own renal biopsies and examination of human endothelial cell cultures exposed to cyclosporine in vitro. Endothelial cells were isolated from human umbilical cords by collagenase digestion and cultured in Medium 199 with Earle's salts plus 20% pooled human serum in the absence of antibiotics. Cultures exposed to cyclosporine (0, 0.4, 1.0, 5.0, 10.0 micrograms/ml) for 0, 3, 7, 10, and 14 days were subsequently fixed in 2% glutaraldehyde in 0.1 M cacodylate buffer. Vascular thrombosis was seen in renal biopsies of cyclosporine- and azathioprine-treated patients but the incidence was the same in both groups. No change in the morphology of endothelial cell cultures was observed until 7 days when an increase in size and number of cytoplasmic inclusions became apparent in both control and cyclosporine-treated cultures. By electron microscopy, these inclusions were identified as secondary lysosomes. Their number and size increased with the length of time in culture but did not appear to correlate with the concentration of cyclosporine in the medium. No other morphologic change was identified. It is concluded that the ap...Continue Reading

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Citations

Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·B F TrumpP C Phelps
Jul 16, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L A Silva-TorresB Zayas
Feb 23, 2010·Molecular and Cellular Biochemistry·Jihee YoonJiho Min

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