Abstract
Cyclosporine (CsA) is a potent agent widely used after organ transplantations and in treatment of various autoimmune disorders. Some patients suffer severe complications including renal and vascular toxicity that are influenced by the degree of endothelial damage. Dysregulation of metalloproteinase (MMP) activity is known to contribute to renal and vascular diseases. To investigate the possible mechanisms of posttransplantation complications in the kidney and vessels by CsA, we examined its effects on metalloproteinases in endothelial cells using human umbilical vein endothelial cells (HUVECs). HUVECs isolated from umbilical cords by collagenase digestion were seeded in 6-well plates at a density of 1 × 10(5) cells/well before treatment with 2-250 μmol/L CsA and a 24-hour incubation. Thereafter we performed gelatin zymography of MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, and MMp-13 to evaluate band density using a luminescent image analyzer system with controls calculated as 100%. MMP-1, MMP-3, MMP-8, MMP-9, and MMP-13 activities were increased after CsA treatment; MMP-1 = 121; MMP-3 = 164; MMP-8 = 133; MMP-9 = 124; and MMP-13 = 121. In contrast, MMP-2 activity was decreased after CsA treatment; MMP-2 = 79. This study showed CsA to act...Continue Reading
References
Jul 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L M Matrisian
Aug 1, 1992·American Journal of Respiratory Cell and Molecular Biology·G Murphy, A J Docherty
Sep 6, 1994·Annals of the New York Academy of Sciences·J F Woessner
Mar 10, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·A FornoniL J Striker
Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B LongoniF Mosca
Dec 5, 2002·Essays in Biochemistry·Yoshifumi Itoh, Hideaki Nagase
May 6, 2003·Circulation Research·Robert Visse, Hideaki Nagase
Jan 27, 2004·Transplantation·Andrea AzzolaMichael Tamm
Sep 15, 2004·Current Opinion in Cell Biology·Joni D Mott, Zena Werb
Dec 17, 2008·Biochemical and Biophysical Research Communications·Wei WangHong Bu
Jan 26, 2011·Acta Dermato-venereologica·Christine D BouboukaAlexandra Katsarou
Jun 23, 2011·Transplantation Proceedings·M KohnoM Iversen
Jun 23, 2011·Transplantation Proceedings·Y TaoZ Luo
Citations
Nov 18, 2014·British Journal of Haematology·Enric Carreras
Mar 16, 2016·Journal of Neurointerventional Surgery·Evan ThielenRamanathan Kadirvel
Mar 14, 2017·Bone Marrow Transplantation·E GavriilakiR A Brodsky
Feb 28, 2017·British Journal of Pharmacology·M P Ward, J P Spiers
Mar 25, 2017·BioMed Research International·Shuqiang WangDi Wu