Effects of cytomegalovirus infection on the mRNA expression of collagens and matrix metalloproteinases in gingival fibroblasts

Journal of Periodontal Research
J E BoteroB Parra

Abstract

The purpose of this in vitro investigation was to study the effects of human cytomegalovirus infection on the mRNA expression for collagens I and III and for matrix metalloproteinases 1 and 2 in gingival fibroblasts. Gingival fibroblasts were experimentally infected with the Towne strain of human cytomegalovirus and the kinetics of expression of mRNA for collagens I and III and for matrix metalloproteinases 1 and 2 was studied at different time-points. Total RNA was isolated at the indicated time, and the reverse transcription-polymerase chain reaction was used to analyze the level of mRNA expression. In addition, gingival specimens were obtained from 14 periodontitis and from three non-periodontitis subjects and mRNA analysis for collagens and metalloproteinases was carried out. Nested polymerase chain reaction was used to determine the presence or absence of human cytomegalovirus in subgingival samples from each subject. The infection of gingival fibroblasts with human cytomegalovirus during a 0-72-h period resulted in progressive reduction in the expression of mRNA for collagens I and III (p < 0.05). A higher concentration of human cytomegalovirus resulted in varying degrees of mRNA reduction, suggesting a virally mediated e...Continue Reading

References

May 1, 1975·Scandinavian Journal of Dental Research·I EricssonH Okamoto
Jan 1, 1988·Environmental and Molecular Mutagenesis·S AbuBakarT Albrecht
Mar 1, 1987·Journal of Oral Pathology·A YamasakiC J Mahan
Oct 1, 1996·European Journal of Oral Sciences·T AibaN Horiuchi
Oct 1, 1996·Oral Microbiology and Immunology·B Parra, J Slots
Dec 1, 1996·Oral Microbiology and Immunology·A Contreras, J Slots
Mar 20, 1999·Transplantation Proceedings·K InkinenI Lautenschlager
Mar 30, 2001·Periodontology 2000·P M BartoldA S Narayanan
May 1, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Kaija InkinenIrmeli Lautenschlager
Jun 24, 2004·Oral Microbiology and Immunology·J Slots
Nov 10, 2004·Oral Diseases·T SorsaT Salo
Jan 10, 2006·Periodontology 2000·P Mark Bartold, A Sampath Narayanan
May 9, 2006·Journal of Clinical Periodontology·Carolina C BituRicardo D Coletta
Nov 2, 2006·Journal of Periodontology·Marcela HernandezJorge Gamonal
Nov 24, 2006·Journal of Dental Research·J Verstappen, J W Von den Hoff
Dec 21, 2006·Emerging Infectious Diseases·Lutz von MüllerThomas Mertens
Apr 4, 2007·Journal of Clinical Periodontology·Lays M SobralRicardo D Coletta
May 15, 2007·Cell Biology International·Gloria Gutiérrez-VenegasLuis Arturo Contreras-Marmolejo

❮ Previous
Next ❯

Citations

Jan 21, 2009·Periodontology 2000·Jørgen Slots
Dec 19, 2009·Periodontology 2000·Jørgen Slots
Dec 17, 2014·Periodontology 2000·Adolfo ContrerasJørgen Slots
Dec 11, 2013·Periodontology 2000·Adolfo ContrerasJørgen Slots
Mar 15, 2016·World Journal of Gastroenterology : WJG·Zhen-Zhai CaiZhan-Xiong Xue

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.