Effects of [D-Ala1] peptide T-NH2 and HIV envelope glycoprotein gp120 on cyclic AMP dependent protein kinases in normal and psoriatic human fibroblasts

The Journal of Investigative Dermatology
C LiapiW B Anderson

Abstract

In addition to acquired immunodeficiency syndrome (AIDS), persons infected with human immunodeficiency virus often develop cutaneous manifestations, including severe psoriasis. In previous studies, we have established that psoriatic fibroblasts and erythrocytes obtained from psoriatic patients exhibit decreased levels of cyclic adenosine monophosphate (cAMP) dependent protein kinase (PKA) activity and of 8-azido-[32P]cAMP binding to the RI and RII regulatory subunits of PKA. Because treatment of patients with peptide T (an octapeptide sequence found in the human immunodeficiency virus envelope glycoprotein gp120) has been observed to result in an improvement in the psoriatic condition, studies were initiated to determine if peptide T and gp120 protein treatment of normal and psoriatic human fibroblasts resulted in any changes in PKA. Exposure of psoriatic fibroblasts to peptide T resulted in a time (4 h to 6 d) and dose [10(-14)-10(-8) M] dependent increase in the levels of 8-azido-[32P]cAMP binding to the RI and RII regulatory subunits of PKA, along with a corresponding increase in PKA activity. Peptide T exhibited a biphasic dose dependent response, with maximal effects on PKA noted at 10(-12)M peptide T. Treatment of normal ...Continue Reading

References

Jan 13, 1978·Science·P Greengard
May 1, 1978·The Journal of Investigative Dermatology·H P BadenJ Kubilus
Oct 1, 1991·Journal of the American Academy of Dermatology·E M FarberD I Wilkinson
May 27, 1989·Lancet·F RaynaudD Evain-Brion
Jun 1, 1989·Journal of Virology·J M HarouseF Gonzalez-Scarano
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M TatenoJ A Levy
Oct 14, 1988·Biochemical and Biophysical Research Communications·F RaynaudD Evain-Brion
Sep 12, 1987·Lancet·M DuvicG W Brewton
Nov 28, 1985·The New England Journal of Medicine·D Casareale, D J Volsky
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D E BrionW Anderson
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Mar 1, 1983·The British Journal of Dermatology·J R BjerkeR Matre
Jan 1, 1983·Methods in Enzymology·U Walter, P Greengard
Jan 1, 1983·Methods in Enzymology·R Roskoski
Jan 1, 1995·Archives of Dermatological Research·T TalmeJ A Marcusson
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y YangJ D Ashwell
Jun 1, 1995·Journal of the American Academy of Dermatology·B L TownsendM Duvic
Apr 1, 1994·Archives of Dermatology·M DuvicS N Lehrman
Jun 28, 1996·Science·R A Weiss

❮ Previous
Next ❯

Citations

Oct 29, 2009·Journal of Peptide Science : an Official Publication of the European Peptide Society·Tran-Chin YangValerie Booth
Jul 9, 2005·Antiviral Research·Maria T PolianovaMichael R Ruff
Oct 21, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·L S RedwineM R Ruff
Mar 25, 2000·Bioorganic & Medicinal Chemistry·S ManfrediniP La Colla
Jan 23, 2014·The Journal of Clinical Endocrinology and Metabolism·Rosemary A ReidEric A Espiner
Sep 1, 2001·Antiviral Research·M R RuffF A Ruscetti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.