PMID: 23591351Apr 18, 2013

Effects of DAPT on proliferation of human dental pulp cells and Notch signaling pathway

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
Xiao-ying ZouXue-jun Gao


To explore whether the γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester (DAPT) could inhibit Notch signaling pathway in human dental pulp cells, and its effects on the proliferation ability of the cells. Human dental pulp cells were primarily cultured from healthy premolars or wisdom teeth extracted intactly. The γ-secretase inhibitor DAPT (5 μmol/L) was added to the culture medium from passage 4 to the end. The cells of passages 4, 8 and 10 were used as check points in this study. The Real-time RT-PCR and RT2 Profiler PCR Array were applied to analyze the expression changes of Notch signaling pathway downstream genes. And the methyl thiazolyl tetrazolium (MTT) method was used to test the proliferation ability of the cells. After DAPT was added, Hes1 gene expression level decreased significantly in the cells of passages 4, 8 and 10 as compared with that of the same passage cells in the control group. The relative gene expression ratio (experimental/control) decreased to 0.20 in the cells of passage 10, and the difference was significant (t=33.143,P=0.001). The PCR Array results of passage 10 dental pulp cells also showed a decline of Notch signaling pathway downstream genes Hey1 and NR...Continue Reading

Related Concepts

Receptors, Notch
Dental Pulp
Tetrazolium Salts
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression
Cell Proliferation
Structure of Wisdom Tooth
NR4A2 gene

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