Effects of defined mixtures of persistent organic pollutants (POPs) on multiple cellular responses in the human hepatocarcinoma cell line, HepG2, using high content analysis screening

Toxicology and Applied Pharmacology
Jodie WilsonL Connolly

Abstract

Persistent organic pollutants (POPs) are toxic substances, highly resistant to environmental degradation, which can bio-accumulate and have long-range atmospheric transport potential. Most studies focus on single compound effects, however as humans are exposed to several POPs simultaneously, investigating exposure effects of real life POP mixtures on human health is necessary. A defined mixture of POPs was used, where the compound concentration reflected its contribution to the levels seen in Scandinavian human serum (total mix). Several sub mixtures representing different classes of POPs were also constructed. The perfluorinated (PFC) mixture contained six perfluorinated compounds, brominated (Br) mixture contained seven brominated compounds, chlorinated (Cl) mixture contained polychlorinated biphenyls and also p,p'-dichlorodiphenyldichloroethylene, hexachlorobenzene, three chlordanes, three hexachlorocyclohexanes and dieldrin. Human hepatocarcinoma (HepG2) cells were used for 2h and 48h exposures to the seven mixtures and analysis on a CellInsight™ NXT High Content Screening platform. Multiple cytotoxic endpoints were investigated: cell number, nuclear intensity and area, mitochondrial mass and membrane potential (MMP) and re...Continue Reading

References

Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B N AmesT M Hagen
Jul 16, 1996·The Science of the Total Environment·P WeiheR White
Oct 20, 1998·Archives of Environmental Contamination and Toxicology·G BachourH Brunn
Jul 27, 1999·Environmental Health Perspectives·A SjödinA Bergman
Mar 8, 2000·Environmental Health Perspectives·E DewaillyR Roy
Feb 13, 2001·Current Opinion in Genetics & Development·Y Shiloh
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·C J Norbury, I D Hickson
Nov 1, 2001·Cell Death and Differentiation·K K KhannaT D Mulhern
Dec 19, 2001·Environmental Health Perspectives·V PerskyUNKNOWN Great Lakes Consortium
Jan 8, 2003·The Journal of Cell Biology·Jean-Ehrland RicciDouglas R Green
Apr 5, 2003·Toxicology Letters·Karl Fent
Dec 24, 2003·Trends in Biotechnology·Vivek C AbrahamJeffrey R Haskins
Apr 20, 2004·Assay and Drug Development Technologies·Kenneth A GiulianoD Lansing Taylor
May 12, 2004·Regulatory Toxicology and Pharmacology : RTP·John L ButenhoffLarry R Zobel
Aug 25, 2004·The Science of the Total Environment·J C Van OostdamJ P Weber
Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Florian WeberMichael Gekle
Feb 3, 2005·Environmental Health Perspectives·Sergio N KuriyamaIbrahim Chahoud
Nov 22, 2005·The Science of the Total Environment·J Van OostdamS Kalhok
Sep 1, 2006·PLoS Medicine·Carsten HeilmannEsben Budtz-Jørgensen
Oct 6, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jing ShaoEvan P Gallagher
Jan 5, 2008·Environmental Health Perspectives·Andreas Kortenkamp
Sep 5, 2008·Environmental Science & Technology·Pal WeihePhilippe Grandjean
Jan 30, 2009·Human Reproduction·Chunyuan FeiJørn Olsen
Feb 13, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Niclas JohanssonHenrik Viberg

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Citations

Apr 4, 2017·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Assem O BarakatMahmoud R Mahmoud
Jul 22, 2017·Environmental Toxicology and Chemistry·Patrícia I MorgadoLuisa Jordao
Aug 31, 2017·Journal of Toxicology and Environmental Health. Part a·Hanne Friis BerntsenKarin Elisabeth Zimmer
Dec 27, 2017·Journal of Applied Toxicology : JAT·Gaston OtarolaSylvain Marcellini
Oct 21, 2017·Toxics·Edna RibeiroSusana Viegas
Nov 22, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Teresa Donato, Laia Tolosa
Jan 17, 2021·Antioxidants·MaríaTeresa Donato, Laia Tolosa
May 21, 2019·Ecotoxicology and Environmental Safety·Donaji J González-MilleRogelio Flores-Ramírez

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