PMID: 3759768Sep 1, 1986Paper

Effects of delayed freezing on content of phosphagens in human skeletal muscle biopsy samples

Journal of Applied Physiology
K Söderlund, E Hultman

Abstract

The concentrations of ATP, phosphocreatine (PCr), creatine, and lactate were determined in muscle biopsy samples frozen immediately or after a delay of 1-6 min. During the delay the samples were exposed to normal air or a gas mixture of 6.5% CO2-93.5% O2. The ATP content was unchanged, but PCr increased significantly from 72 mmol after rapid freezing to 85 mmol X kg dry muscle-1 during the 1st min in air. The lactate concentration increased (2.8 to 5.2 mmol X kg-1). If muscles were made anoxic by circulatory occlusion for 4-6 min before sampling, no increase in PCr was observed. Direct homogenization of fresh tissue in perchloric acid gave the same ATP, PCr, and lactate contents as frozen samples. It is concluded that the ATP and PCr contents in muscle are unaffected by freezing but that the biopsy procedure activates the energy utilization processes resulting in PCr decrease. It is suggested that the muscle PCr content after a 1-min delay in tissue freezing corresponds to the level in resting fresh muscle.

Citations

Aug 13, 1999·Journal of Applied Physiology·T M RobinsonP L Greenhaff
Nov 19, 2005·Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée·David J Baker, Russell T Hepple
Jul 8, 2018·Thorax·Zudin A PuthuchearyHugh E Montgomery
Dec 12, 2001·Medicine and Science in Sports and Exercise·M A TarnopolskyS M Phillips
Aug 29, 2002·The American Journal of Clinical Nutrition·Mark D HaubWayne W Campbell
Oct 13, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·H E KanA Heerschap
Aug 1, 1996·NMR in Biomedicine·H J LambA de Roos
Aug 1, 1995·Journal of Internal Medicine·G RonquistA Waldenström
Jan 15, 2004·Intensive Care Medicine·Inga TjäderJan Wernerman
Dec 9, 1998·The American Journal of Physiology·G R SteengeP L Greenhaff
Dec 28, 2019·International Journal of Molecular Sciences·Spencer G MillerJeffrey J Brault
Jan 7, 2003·Journal of Applied Physiology·Jeffrey J BraultRonald L Terjung
Mar 1, 1991·Pflügers Archiv : European journal of physiology·M Bergström, E Hultman
Nov 30, 2007·International Journal of Chronic Obstructive Pulmonary Disease·Gun FaagerPer Jakobsson
Sep 1, 1995·European Journal of Obstetrics, Gynecology, and Reproductive Biology·K WedenbergA Waldenström
Mar 17, 1988·Biochimica Et Biophysica Acta·J ChaoL Chao

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