PMID: 15226593Jul 1, 2004Paper

Effects of delipidation and oxygen concentration on in vitro development of porcine embryos

The Journal of Reproduction and Development
Akihiro YonedaTomomasa Watanabe

Abstract

The effects of delipidation and the oxygen (O(2)) concentration in the atmosphere during culture on in vitro development and H(2)O(2) content were investigated in porcine in vivo fertilized embryos and embryos after in vitro maturation and in vitro fertilization (IVM/IVF embryos). There was no significant difference in the developmental rates to the blastocyst stage between the intact and delipidated IVM/IVF embryos. However, the mean number of cells in blastocysts derived from delipidated IVM/IVF embryos (19.8 +/- 0.8 cells) was significantly smaller than that from intact embryos (24.2 +/- 1.2 cells). Although there were no significant differences in the developmental rates to the blastocyst stage of intact and delipidated IVM/IVF embryos between the cultures under 5% O(2) and 20% O(2), the developmental rate of intact IVM/IVF embryos cultured under 5% O(2) (27.1%) was significantly higher than that of the delipidated embryos cultured under 20% O(2) (19.3%). On the other hand, there was no difference in the developmental rate to the blastocyst stage between in vivo fertilized embryos cultured under 5% O(2) and 20% O(2). Hydrogen peroxide (H(2)O(2)), one of the reactive oxygen species (ROS), is thought to cause damage to embryo...Continue Reading

References

Jan 1, 1978·Biochemical Pharmacology·C J Fowler, B A Callingham
Jan 1, 1992·Free Radical Biology & Medicine·Y GotoT Mori
Mar 1, 1991·Journal of Animal Science·D R HagenN L First
May 1, 1990·Journal of Reproduction and Fertility·R M PettersA E Archibong
Jul 1, 1990·Journal of Reproduction and Fertility·J G ThompsonH R Tervit
Jul 1, 1989·Biology of Reproduction·R J AitkenS Fishel
Mar 30, 1995·Nature·H NagashimaM B Nottle
Oct 1, 1994·Biology of Reproduction·H NagashimaM B Nottle
Nov 1, 1993·Journal of Reproduction and Fertility·B Fischer, B D Bavister
Jul 1, 1993·Free Radical Biology & Medicine·Y GotoM Nakano
Aug 1, 1998·Biology of Reproduction·Z MachátyR S Prather
May 4, 2000·Journal of Reproduction and Fertility·T G McEvoyB K Speake
Oct 21, 2000·Human Reproduction·J W Catt, M Henman
Aug 23, 2001·Current Biology : CB·D A Brown
Dec 26, 2001·Biology of Reproduction·Koji YoshiokaShokichi Iwamura

❮ Previous
Next ❯

Citations

Oct 21, 2015·Journal of Pineal Research·Xue-Ming ZhaoHua-Bin Zhu
Mar 16, 2007·Cryobiology·Y DuG Vajta
Aug 13, 2009·Reproduction in Domestic Animals = Zuchthygiene·R G SturmeyT G McEvoy
Jul 20, 2016·PloS One·Ji Hye LeeMin Kyu Kim
Oct 27, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Joseph Saragusty, Amir Arav
Jan 30, 2007·Reproduction, Fertility, and Development·Gábor VajtaZoltán Macháty
Oct 18, 2020·International Journal of Molecular Sciences·Tania García-MartínezTeresa Mogas
Feb 3, 2021·International Journal of Molecular Sciences·Franciele LanzariniMateus José Sudano

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.