Effects of dendritic polyglycerol sulfate on articular chondrocytes

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Tobias SchneiderGundula Schulze-Tanzil

Abstract

Inflammatory processes driven by cytokines play a crucial role during osteoarthritis (OA) progression. Dendritic polyglycerol sulfate (dPGS) was analyzed in vitro for its effects on articular chondrocytes, cartilage and cytokines involved in the OA process. The metabolic activity of cultured human articular chondrocytes stimulated for 24 h with dPGS (10(-3)-10(-6) mol/L) was monitored using AlamarBlue(®) assay. The dPGS uptake was studied using fluorescence labeled nanoparticles. Further, chondrocytes were either treated with 10(-6) M dPGS, TNFα (10 ng/mL) alone or with a combination of both. The influence on extracellular matrix components, pro- and anti-inflammatory cytokines, matrix metalloproteinase (MMP)1 and the anaphylatoxin receptor C3aR was analyzed by RTD-PCR, flow cytometry and ELISA. Even at higher dosages (10(-3) mol/L), dPGS did not influence chondrocytes viability. Uptake of dPGS was successfully monitored in human articular chondrocytes and synovial fibroblasts, penetration into cartilage chips was up to ~50 µm. Cellular treatment with dPGS had no effect on synthesis of pro-inflammatory cytokines TNFα and IL-6, but expression of the anti-inflammatory IL-10 was upregulated. Cotreatment with TNFα and dPGS reduced ...Continue Reading

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Citations

Dec 17, 2015·BMC Musculoskeletal Disorders·Tobias SchneiderGundula Schulze-Tanzil
Jun 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sabine ReimannRainer Haag
Apr 11, 2019·Polymers·Nadine RadesRainer Haag
Jun 27, 2020·Angewandte Chemie·Katharina AchaziGerd Multhaup
Feb 20, 2021·Journal of Experimental Pharmacology·Gundula Schulze-Tanzil

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