PMID: 8952956Dec 1, 1996Paper

Effects of density and gating of delayed-rectifier potassium channels on resting membrane potential and its fluctuations

The Journal of Membrane Biology
S MaromE Braun

Abstract

The aim of this study is to evaluate directly, using a reduced experimental system, the nature of interactions between voltage-gated potassium channels and the resting membrane potential. Xenopus oocytes were injected with various concentrations of cRNA coding for a delayed-rectifier potassium channel Shaker-IR. The effects of the density and kinetics of the expressed channels on resting membrane potential is explored in isolated ("inside-out") patches. The channel density is given in terms of maximal conductance (Gmax), measured from the maximal slope of the I-V curve under voltage clamp conditions. The capacitance of the experimental setup is approximately 1 pF. At high channel densities (Gmax > 10 pA/mV) the mean membrane potential is stabilized at approximately -60 mV. This resting membrane potential is more than 35 mV positive to the reversal potential for potassium ions under the same experimental conditions. Analyses of voltage clamp experiments indicate that at high channel densities the mean membrane potential is determined by the rates of channel activation and deactivation, but is not affected by the rates involved in the process of slow (C-type) inactivation. In contrast, at lower channel densities membrane potentia...Continue Reading

Citations

Feb 17, 2000·Trends in Neurosciences·J A WhiteA R Kay
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Igor Goychuk, Peter Hänggi
Nov 23, 2000·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·I Goychuk, P Hänggi
Aug 11, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Igor GoychukSalvador Miret-Artés
Jan 24, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anne Marie F SalapatekNicholas E Diamant

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