PMID: 11926234Apr 3, 2002Paper

Effects of dental resins on TNF-alpha-induced ICAM-1 expression in endothelial cells

Journal of Dental Research
E L KostoryzD M Yourtee

Abstract

Many reports have demonstrated inflammation after the placement of dental restorations. To explain this side-effect, we studied a biomarker in the inflammatory response. The intercellular adhesion molecule-1 (ICAM-1) is a key mediator for recruitment of leukocytes to the site of inflammation. Therefore, we investigated whether methacrylates (a BISGMA-based dental resin, BISGMA, and MAA) and Cyracure UVR 6105, an epoxy monomer, could alter ICAM-1 expression in unstimulated and TNF-alpha-stimulated endothelial cells. Six-well plates with monolayers of human umbilical vein cells, ECV 304 (ATCC CRL 1998), were exposed to TNF-alpha (1 ng/mL) in the presence and absence of subtoxic and TC50 doses of chemicals for 24 hrs at 37 degrees C/5% CO2. Several doses of TNF-alpha (0.5-2 ng/mL) were coincubated with 100 microL of undiluted aqueous dental resin extracts. Cells were harvested and stained with mAB FITC-conjugated anti-human ICAM-1 (CD54). ICAM-1 expression was measured by flow cytometry. Cells expressed basal levels of ICAM-1, which was up-regulated by TNF-alpha but was not changed by all samples studied. Except for UVR 6105, the methacrylates significantly decreased ICAM-1 expression in TNF-alpha-stimulated cells. These findings ...Continue Reading

References

Sep 1, 1992·Advances in Dental Research·H R Stanley
Aug 1, 1997·Journal of Biomedical Materials Research·I C WatahaD N Fang
Apr 16, 1998·Journal of Dental Research·F MillichC S Pinzino
Jun 23, 2000·Dental Materials : Official Publication of the Academy of Dental Materials·E L KostoryzD M Yourtee
Jul 25, 2000·Biomaterials·D A TilbrookM Braden

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Citations

Oct 30, 2009·International Journal of Molecular Sciences·Athina BakopoulouPavlos Garefis
Feb 11, 2009·Dental Materials : Official Publication of the Academy of Dental Materials·M AnagnostouC Tesseromatis
Jul 26, 2005·Dental Materials : Official Publication of the Academy of Dental Materials·Homa Darmani, Ahmad S Al-Hiyasat
Oct 27, 2004·Journal of Dental Research·G SpagnuoloS Rengo
Aug 25, 2016·Journal of Applied Oral Science : Revista FOB·Daniele Lucca LongoLéa Assed Bezerra da Silva
Apr 24, 2003·Journal of Dental Research·E L KostoryzD M Yourtee
Mar 5, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Dragica ManojlovicBiljana Nikolić
Oct 6, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M Goël BrackettJ C Wataha
Apr 20, 2004·Journal of Oral Rehabilitation·S Bouillaguet, J C Wataha
Dec 21, 2010·Dental Materials : Official Publication of the Academy of Dental Materials·Dragica ManojlovicVesna Miletic

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