PMID: 9166755Jun 1, 1997Paper

Effects of depolarization and NMDA antagonists on the role survival of cerebellar granule cells: a pivotal role for protein kinase C isoforms

Journal of Neurochemistry
W W LinD M Chuang

Abstract

Primary cultures of cerebellar granule cells (CGCs) grown in high-K+ (25 mM; K25) medium progressively differentiate in vitro. Differentiation is noticeable after 3-4 days in vitro (DIV) and reach a mature stage after 8 DIV. Longer cultivation of CGCs (>13 DIV) triggers the processes of spontaneous cell death. However, if cultured in normal physiological K concentration (5 mM; K5), a significant proportion of the cells dies by the end of the first week in culture. To address the role of protein kinase C (PKC) in the development of CGCs, we measured the kinase activity as well as the protein level of the kinase isoforms. As the K25 CGC culture proceeded, the PKC activity time-dependently increased by 3.2-fold, reaching a steady state at 8 DIV. Western blot analysis using PKC isoform-specific antibodies revealed an increase in levels of PKC alpha, gamma, mu, lambda, and iota from 2 to 8 DIV. A slight increase or decrease at 4 DIV was observed for PKC epsilon and betaII, respectively, whereas no significant change was observed for betaI. The isoforms of delta, theta, eta, and zeta were not detected. Comparing the 14 DIV cultures with the 10 DIV cultures, the immunoreactivities of PKC iota and epsilon were decreased, those of PKC a...Continue Reading

Citations

Jan 16, 2008·Cellular and Molecular Neurobiology·Sarah L RinglerChristopher P Turner
Mar 21, 2006·Cell Biology and Toxicology·S-Z XuB Rajanna
Feb 28, 2002·The Journal of Pharmacology and Experimental Therapeutics·David R Lynch, Rodney P Guttmann
Oct 17, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Maike Hartlage-RübsamenSteffen Roßner
Feb 13, 2001·Biochemical and Biophysical Research Communications·J H Yang, P R Kodavanti
Oct 11, 2005·Neurotoxicology·Shang-Zhi Xu, Bettaiya Rajanna

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