PMID: 6798768Oct 1, 1981

Effects of detergents and organic solvents on rat liver microsomal UDP-glucuronosyltransferase activity towards phenolic substrates

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
H P Illing, E S House

Abstract

1. The effects of several detergents (Brij 58, deoxycholate and Lubrol 12A9) and ether on the initial rate of UDP-glucuronosyltransferase activity towards fixed concentrations of five phenolic acceptor substrates of widely different octanol-buffer (pH 7.4) partition coefficient have been compared with those observed in non-activated and Triton X-100-and n-pentane-activated rat liver microsomes. 2. Enzymic activity was dependent on the lipid-solubility of acceptor substrate. Each activator, except Triton X-100, enhanced enzymic activity towards all substrates by a similar factor, which was independent of the octanol-buffer partition coefficient. For Triton X-100 microsomes, the activation was also partition-dependent. 3. The highest activation factor was seen with ether. Pre-incubation of ether-activated microsomes for 30 min at 37 degrees C before assay resulted in inactivation of the enzyme towards more water-soluble substrates. Tryptic digestion (30 min at 37 degrees C) of the ether-activated microsomes resulted in marked reduction of enzyme activity towards all substrates. 4. Ether, and the two detergents, Brij 58 and Lubrol 12A9, released small amounts of protein (5-12% total present); both detergents also released some (8-...Continue Reading

References

Jul 1, 1979·European Journal of Clinical Pharmacology·T J MalingC T Dollery
Mar 15, 1977·FEBS Letters·J Wilkinson, T Hallinan
Aug 1, 1977·Journal of Steroid Biochemistry·G W Lucier, O S McDaniel
May 15, 1975·Biochemical Pharmacology·W R Notten, P T Henderson
Aug 1, 1971·Archives of Biochemistry and Biophysics·G W LucierH B Matthews
Dec 1, 1970·The Biochemical Journal·J A Meuwissen, K P Heirwegh
Feb 15, 1980·Biochemical Pharmacology·K W BockK L Platt
Feb 1, 1980·Biochemical Society Transactions·H P Illing
Jun 15, 1981·Biochemical Pharmacology·I Okulicz-KozarynV Loppinet

Related Concepts

Thesit
Pentane
Ceteareth 20
Deoxycholic Acid, Sodium Salt, 12beta-Isomer
Synthetic Detergent
Ethyl ether
Microsomes, Liver
Isopentanes
Phenols
Vigilon

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