Effects of dexamethasone on Muc5ac mucin production by primary airway goblet cells

American Journal of Physiology. Lung Cellular and Molecular Physiology
Wenju LuK Chul Kim

Abstract

Mucus hypersecretion associated with airway inflammation is reduced by glucocorticoids. Two mechanisms of glucocorticoid-mediated inhibition of mucus production have been proposed, direct inhibition of mucus production by airway epithelial cells and indirectly through inhibition of proinflammatory mediators that stimulate mucus production. In this study, we examined the effect of dexamethasone (DEX) on mRNA expression and synthesis of MUC5AC by A549 human lung adenocarcinoma cells as well as Muc5ac and total high-molecular-weight (HMW) mucins by primary rat tracheal surface epithelial (RTSE) cells. Our results showed that in primary RTSE cells, DEX 1) dose dependently suppressed Muc5ac mRNA levels, but the levels of cellular Muc5ac protein and HMW mucins were unaffected; 2) did not affect constitutive or UTP-stimulated mucin secretion; 3) enhanced the translation of Muc5ac; and 4) increased the stability of intracellular Muc5ac protein by a mechanism other than the inhibition of the proteasomal degradation. In A549 cells, however, DEX suppressed both MUC5AC mRNA levels and MUC5AC protein secretion in a dose-dependent manner. We conclude that whereas DEX inhibits the levels of Muc5ac mRNA in primary RTSE cells, the levels of Muc...Continue Reading

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Citations

Jan 19, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yusheng LiK Chul Kim
Jan 24, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yong Sung ParkKwang Chul Kim
Oct 22, 2005·American Journal of Respiratory Cell and Molecular Biology·Yajun ChenMary C Rose
Feb 4, 2006·American Journal of Respiratory Cell and Molecular Biology·Judith A VoynowMary C Rose
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Dec 15, 2007·American Journal of Respiratory Cell and Molecular Biology·Keiko UenoK Chul Kim
Feb 3, 2012·American Journal of Respiratory Cell and Molecular Biology·Yoshiyuki KyoKwang Chul Kim
Jul 17, 2012·American Journal of Respiratory Cell and Molecular Biology·Yajun ChenMary C Rose
Jan 22, 2011·Proteome Science·Mehboob AliK Chul Kim
Dec 10, 2008·Archives of Medical Research·Lei ChenFu-Qiang Wen
Aug 22, 2014·European Journal of Immunology·Zhengtu LiWenju Lu
Feb 16, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jin Yong AnEui-Bae Jeung
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Dec 15, 2010·Pulmonary Pharmacology & Therapeutics·Lisa SprengerHans-Peter Hauber

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