Effects of Dexamethasone on Satellite Cells and Tissue Engineered Skeletal Muscle Units

Tissue Engineering. Part a
Brian C SyverudLisa M Larkin

Abstract

Tissue engineered skeletal muscle has potential for application as a graft source for repairing soft tissue injuries, a model for testing pharmaceuticals, and a biomechanical actuator system for soft robots. However, engineered muscle to date has not produced forces comparable to native muscle, limiting its potential for repair and for use as an in vitro model for pharmaceutical testing. In this study, we examined the trophic effects of dexamethasone (DEX), a glucocorticoid that stimulates myoblast differentiation and fusion into myotubes, on our tissue engineered three-dimensional skeletal muscle units (SMUs). Using our established SMU fabrication protocol, muscle isolates were cultured with three experimental DEX concentrations (5, 10, and 25 nM) and compared to untreated controls. Following seeding onto a laminin-coated Sylgard substrate, the administration of DEX was initiated on day 0 or day 6 in growth medium or on day 9 after the switch to differentiation medium and was sustained until the completion of SMU fabrication. During this process, total cell proliferation was measured with a BrdU assay, and myogenesis and structural advancement of muscle cells were observed through immunostaining for MyoD, myogenin, desmin, and...Continue Reading

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Citations

Mar 2, 2018·Tissue Engineering. Part C, Methods·Brian C SyverudLisa M Larkin
Apr 14, 2018·Tissue Engineering. Part B, Reviews·Molly N Pantelic, Lisa M Larkin
Sep 28, 2017·Tissue Engineering. Part C, Methods·Brian C SyverudLisa M Larkin
Aug 16, 2017·Tissue Engineering. Part C, Methods·Brian C SyverudLisa M Larkin
Aug 31, 2019·Tissue Engineering. Part a·Brittany L RodriguezLisa M Larkin
Jun 21, 2019·Regenerative Engineering and Translational Medicine·Brittany L RodriguezLisa M Larkin
Feb 13, 2019·BMC Veterinary Research·Iwona Otrocka-DomagałaMichał Gesek
Feb 6, 2020·Tissue Engineering. Part a·Stoyna S NovakovaLisa M Larkin
Aug 1, 2020·Food Science of Animal Resources·Kwang-Hwan ChoiChang-Kyu Lee
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Kwang-Hwan ChoiChang-Kyu Lee
Nov 19, 2020·Tissue Engineering. Part a·Olga M WroblewskiLisa M Larkin
Dec 24, 2021·Tissue Engineering. Part a·Brittany Lynn RodriguezLisa Marie Larkin

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Methods Mentioned

BETA
delamination

Software Mentioned

ImageJ
Image J
LabVIEW
R

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