PMID: 2430932Nov 1, 1986

Effects of dextran-bound inhibitors on carbonic anhydrase activity in isolated rat lungs

Journal of Applied Physiology
T A HemingE D Crandall

Abstract

Effects of macromolecular Prontosil-dextran inhibitors (PD) on carbonic anhydrase (CA) activity in isolated rat lungs were studied. Isolated lungs were perfused with Krebs-Ringer bicarbonate (KRB) solutions containing no inhibitor, PD 100,000 (mol wt 100,000), PD 5,000 (mol wt 5,000), or low-molecular-weight inhibitors (Prontosil or acetazolamide). The time course of effluent perfusate pH equilibration was measured in a stop-flow pH electrode apparatus. Pulmonary CO2 excretion (Vco2) was monitored by continuously recording expired CO2 concentration. The lungs were ventilated with room air and perfused at 37 degrees C with KRB prebubbled with 5% CO2- 20% O2- 75% N2. The results obtained show that both the low-molecular-weight inhibitors and PD's caused postcapillary pH disequilibria (delta pH) in effluent perfusate. However, only acetazolamide and Prontosil caused a reduction in Vco2. These results suggest that there is an intravascular CA, presumably associated with endothelial cell membranes, that is accessible to all inhibitors used and is responsible in part for equilibration of the CO2- HCO3- -H+ reactions in the perfusate but, under the conditions used, does not affect CO2 excretion; and there is an extravascular (possibly...Continue Reading

Citations

Jan 25, 2000·American Journal of Physiology. Cell Physiology·Thomas E DeCoursey
Dec 25, 2002·Medicinal Research Reviews·Claudiu T SupuranAngela Casini
Nov 1, 1987·Equine Veterinary Journal·R C Harris
Mar 29, 2003·Physiological Reviews·Thomas E DeCoursey
Aug 9, 2019·International Journal of Molecular Sciences·Rossana Occhipinti, Walter F Boron
Apr 4, 2000·Physiological Reviews·C Geers, G Gros
Mar 5, 1998·The American Journal of Physiology·Y H Lien, L W Lai

Related Concepts

Sulfamidochrysoidine hydrochloride
Acetazolamide Sodium, (Sterile)
Azo Compounds
Carbonic Anhydrase Inhibitors
Saviosol
Hydrogen-Ion Concentration
Lung
C.I. Solvent Yellow 1
Perfusion
Pulmonary Gas Exchange

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